A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells

doi:10.6023/cjoc201808035

Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (3): 697-702.DOI: 10.6023/cjoc201808035 Previous Articles Next Articles

Special Issue: 荧光探针-生物传感合辑

Articles

一种黄酮荧光探针对肼的识别及细胞成像

鞠志宇^a, 舒朋华^a, 谢智宇^a, 蒋雨晴^a, 陶伟杰^a, 许志红^a,b

a 许昌学院化学化工学院许昌市化学生物传感与检测重点实验室许昌 461000;
b 郑州大学化学与分子工程学院郑州 450052

收稿日期:2018-08-28 修回日期:2018-11-03 发布日期:2018-11-26
通讯作者: 鞠志宇, 许志红 E-mail:juz1696@163.com;xuzhihong1980@yahoo.com
基金资助:
河南省科技计划项目（Nos.182102311108，182102310066）、河南省高校科技创新人才计划（No.15HASTIT002）、国家自然科学基金（Nos.21702178，U1304202）资助项目.

A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells

Ju Zhiyu^a, Shu Penghua^a, Xie Zhiyu^a, Jiang Yuqing^a, Tao Weijie^a, Xu Zhihong^a,b

a Xuchang Municipality Key Laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University, Xuchang 461000;
b College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052

Received:2018-08-28 Revised:2018-11-03 Published:2018-11-26
Contact: 10.6023/cjoc201808035 E-mail:juz1696@163.com;xuzhihong1980@yahoo.com
Supported by:
Project supported by the Science and Technology Project of Henan Province (Nos. 182102311108, 182102310066), the Program for Science and Technology Innovation Talents in Universities of Henan Province (No. 15HASTIT002), and the National Natural Science Foundation of China (Nos. 21702178, U1304202).

1. A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells.PDF(518KB)

Abstract

A 3-hydroxyflavonoid esters HFBA was synthesized by the reaction of 2-hydroxyacetophenone with p-methyl benzaldehyde, and its structure was characterized by NMR and high resolution mass spectrometer. The recognition behaviors of HFBA to hydrazine were investigated and the results show that HFBA exhibits good selectivity and sensitivity to N₂H₄ with fast response and good anti-interference ability in DMSO/PBS buffer (pH=7.4). Furthermore, the detection limit of HFBA for hydrazine was estimated to be 0.11 μmol/L, and the probe HFBA was successfully applied for the imaging of hydrazine in living cells.

Key words: flavone, fluorescence, hydrazine, biological imaging

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Ju Zhiyu, Shu Penghua, Xie Zhiyu, Jiang Yuqing, Tao Weijie, Xu Zhihong. A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells[J]. Chin. J. Org. Chem., 2019, 39(3): 697-702.

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一种黄酮荧光探针对肼的识别及细胞成像

A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells

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