Terpenoids from the Leaves of Turpinia arguta and Their Bioactivities

doi:10.6023/cjoc202507018

Abstract

A phytochemical investigation of the 90% MeOH extract of the leaves of Turpinia arguta led to the identification of 22 structurally diverse terpenoids, including two new ones: argutacid A (1, a 15-oxo-17-norabietane-type diterpenoid) and argutacid B (2, a labdane-type diterpenoid). Their structures were determined using spectroscopic techniques including mass spectrometry, one-dimensional/two-dimensional nuclear magnetic resonance (NMR) spectra, and electronic circular dichroism (ECD). Compounds 4, 7, 8, 10-14, 16-20, and 22 are reported from T. arguta for the first time. The isolated compounds were evaluated for their anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 cells by suppressing nitric oxide (NO) production. Two oleanolic acid derivatives, pomolic acid (19) and 3β-cis-p-coumaroyloxy-2α,23-dihydroxyolean acid (22), were found to have anti-inflammatory activities with IC₅₀ values of 11.3 and 19.1 μM, respectively. Such an anti-inflammatory effect of 22 was reported for the first time. Furthermore, isoyarumic acid (9, a ursolic acid derivative) exhibited moderate cytotoxic effect against MDA-MB-231 cells, with an IC₅₀ value of 17.6 μM.

Key words: Turpinia arguta, Diterpenes, Argutacid A, Argutacid B, Triterpenoids, Anti-inflammatory activity, Cytotoxicity

Cite this article

Zhe-Lu Jiang, Ze-Yu Zhao, Chun-Jing Chi, Jiang-Chuan Yu, Li-Hong, Yan, Jin-Feng Hu. Terpenoids from the Leaves of Turpinia arguta and Their Bioactivities[J]. Chinese Journal of Organic Chemistry, doi: 10.6023/cjoc202507018.

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