化学学报 ›› 2019, Vol. 77 ›› Issue (12): 1239-1249.DOI: 10.6023/A19080305 上一篇    下一篇

综述

稀土纳米晶用于近红外区活体成像和传感研究进展

熊麟, 凡勇, 张凡   

  1. 复旦大学化学系 聚合物工程国家重点实验室 上海 200433
  • 收稿日期:2019-08-16 出版日期:2019-12-15 发布日期:2019-09-18
  • 通讯作者: 张凡 E-mail:zhang_fan@fudan.edu.cn
  • 作者简介:熊麟,博士后,2004年获得上海交通大学工学学士学位,2007年获得上海硅酸盐研究所材料物理与化学专业硕士学位,2017年获得澳大利亚阿德莱德大学纳米材料与纳米医学专业博士学位.2017年9月加入复旦大学化学系,主要研究领域为纳米材料的设计与合成,及其在药物递送和生物传感中的应用;凡勇,复旦大学化学系青年研究员,2009年获得西安交通大学理学学士学位,2015年获得清华大学物理系理学博士学位,2015~2018年复旦大学化学系博士后,2019年1月加入复旦大学化学系,主要研究领域包括功能性荧光纳米材料、荧光介观材料的设计与合成及其在医学成像、疾病诊断和治疗中的应用;张凡,复旦大学化学系教授,博士生导师,国家杰出青年基金获得者,教育部青年长江学者,中组部青年拔尖人才.2008年获得复旦大学化学系理学博士学位,2008~2010年美国加州大学圣芭芭拉分校化学与生物化学系博士后.2010年8月加入复旦大学化学系,主要研究领域包括生物纳米技术及生物分析、药物存储与释放,体内与体外生物成像等.
  • 基金资助:
    项目受国家自然科学基金杰出青年基金(No.21725502)、国家重大研究计划项目(No.2017YFA0207303)和上海市基础研究重大项目(No.17JC1400100)资助.

Research Progress on Rare Earth Nanocrystals for In Vivo Imaging and Sensing in Near Infrared Region

Xiong Lin, Fan Yong, Zhang Fan   

  1. State Key Laboratory of Molecular Engineering of Polymers, Department of Chemistry, Fudan University, Shanghai 200433
  • Received:2019-08-16 Online:2019-12-15 Published:2019-09-18
  • Supported by:
    Project supported by National Science Fund for Distinguished Young Scholars (No. 21725502), the National Key R&D Program of China (No. 2017YFA0207303), the Key Basic Research Program of Science and Technology Commission of Shanghai Municipality (No. 17JC1400100).

稀土纳米晶具有丰富的激发和发射波长,良好的化学和光稳定性、大Stokes位移等特点.近年来,稀土纳米晶在生物活体成像与传感领域的应用研究取得了迅速进展.通过纳米尺度的材料设计与合成,可以对稀土纳米晶的荧光效率、波长、寿命等光学性质,以及生物相容性、靶向性、响应性等生化性质进行精细调控,使其更好地适应于活体深组织的成像与分析.先概述活体荧光成像的技术特点与要求,然后介绍稀土纳米晶的一般组成、光学性质和荧光机理,随后详细讨论对稀土纳米晶光学和生化性质进行调控的方法,着重展示这些材料的设计和修饰在生物成像与传感领域的一些最新应用.通过总结最近的研究成果,期望能够为下一步的研究提供一些参考思路,以推进基于稀土纳米晶的生物成像与传感技术的临床转化和应用.

关键词: 稀土纳米晶, 生物成像, 生物传感, 近红外, 荧光

In vivo imaging and sensing play a critical role in modern biological and medical research. Compared with other techniques such as computed tomography (CT), positron emission tomography (PET) and nuclear magnetic resonance (NMR), fluorescence imaging and analysis are featured by fast feedback, high sensitivity, and high spatiotemporal resolution. Especially, the application of near infrared (NIR) light as both excitation and emission signals provides increased tissue penetration and improved imaging quality and sensitivity due to reduced light scattering and auto-fluorescence. Among various materials investigated for in vivo imaging and bio-sensing, lanthanide-based nanocrystals display rich excitation/emission wavelengths in the NIR range, good photo and chemical stability, large Stokes shifts. In recent years, the research on lanthanide-based nanocrystals for in vivo imaging and sensing has seen rapid progress. Through nanoscale material design and synthesis, it is possible to fine tune the optical properties of lanthanide-based nanocrystals. By properly choosing different lanthanide ions as activators and sensitizers, multiple excitation/emission wavelengths can be obtained. The careful design of core-shell structure of nanocrystals enables improved fluorescence efficiency and tailorable fluorescence life time through controlled energy transfer. On the other side, the surface of lanthanide-based nanocrystals can be modified through coating, absorption or ligand exchange to enhance the biocompatibility, targeting capability, and bio-responsiveness. Taking advantage of this high flexibility and versatility, there are great opportunities for these lanthanide-based nanocrystals in various in vivo imaging and sensing applications. This review first outlines the general technique requirements for in vivo imaging and sensing. Then, the composition, synthesis and basic properties of lanthanide-based nanocrystals are briefly introduced. Subsequently, the routes for tailoring the optical and biochemical properties of lanthanide-based nanocrystals are discussed in detail, with an emphasis on the material designs and surface modifications for in vivo imaging and analysis. It is expected that this work will inspire new ideas for accelerating the clinic translation of rare earth nanocrystals-based imaging and sensing techniques.

Key words: rare earth nanocrystals, bio-imaging, bio-sensing, near infrared, fluorescence