关键词: 锇胺, 脱氧鸟苷酸, 咪唑, 磷酸氢二钠, 核苷酸, 探针

Interaction between dGMP or its one-binding-site analogues-imidazole, NaH~2PO~4, Na~2HPO~4 and the probe [trans-en~2Os(η^2-H~2)D~2O]^2^+ were studied. The results suggested that the coordination between dGMP and the probe was not the simple sum of the coordination of probe with imidazole and that with NaH~2PO~4 or Na~2HPO~4. The solution chemistry and the dynamics of anticancer agents Me~2SnCl~2, Bu~2SnCl~2 competing for the binding sites of dGMP with the probe at different concentrations were studied using 1^H NMR and UV-stopped flow instruments. Binding sites, equilibrium constants and the dynamic results of the probe and the anticancer agents with dGMP were thus obtained. Some reaction mechanisms is also proposed.

Key words: IMIDAZOLE, DISODIUM HYDROGEN PHOSPHATE, NUCLEOTIDES, PROBES