Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (11): 1315-1321.DOI: 10.6023/A1111012 Previous Articles     Next Articles

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新型石墨烯/金/功能导电高分子/过氧化氢生物传感器的制备及应用

夏前芳, 黄颖娟, 杨雪, 李在均   

  1. 江南大学化学与材料工程学院 无锡 214122
  • 收稿日期:2011-11-01 修回日期:2012-04-12 出版日期:2012-06-14 发布日期:2012-04-12
  • 通讯作者: 李在均 E-mail:zaijunli@263.net
  • 基金资助:

    国家自然科学基金(No. 21176101)、国家科技支撑计划(No. 2011BAK10B03)、浙江省自然科学基金(No. Y4100729)、江苏省“青蓝工程”和浙江赞宇基金资助项目.

Preparation and its Application of Novel Graphene/Gold/Functional Conducting Polymer/Hydrogen Peroxide Biosensor

Xia Qianfang, Huang Yingjuan, Yang Xue, Li Zaijun   

  1. School of Chemical and Materials Engineering, Jiangnan University, Wuxi 214122
  • Received:2011-11-01 Revised:2012-04-12 Online:2012-06-14 Published:2012-04-12
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21176101), the National Science and Technology Support Plan (No. 2011BAK10B03), Qing Lan Project, the Natural Science Foundation of Zhejiang Province (No. Y4100729) and Zanyu Science Foundation of Zhejiang ZanYu Technology Limited Company.

Immobilizations of the graphene-based materials and enzyme are very important to electrochemical properties and use of the biosensor. In this work, a 0.005 mg/mL of graphite oxide and 0.005 mM of chlorauric acid were in sequence electrodeposited on the surface of gold electrode with potentiostatic elec-trolysis. After above procedure was repeated for 20 cycles, 2,5-di-(2-thienyl)-1-pyrrole-1-(p-benzoic acid) was electropolymerized on the modified electrode by cyclic voltammetry and finally formed functional conducting polymer film containing carbonyl groups on surface of the graphene/gold nanocomposite. To prepare hydrogen peroxide biosensor, the horseradish peroxidase was subsequently connected covalently to the film with DHC/NHS as activator. Research results indicated that the graphene/gold nanocomposite obtained using alternating electro-deposition has excellent dispersivity. The biosensor based on the material offers remarkable catalysis performance to the redox of hydrogen peroxide on the electrode surface. Current response of the sensor increases linearly with the increasing concentration of hydrogen peroxide over the range from 2 nM to 200 nM, with a correlation coefficient (R2) of 0.9996. The detection limit was found to be 0.67 nM (S/N=3). The sensitivity is more than other sensor reported in the literatures. In addition, covalent immobilization of the enzyme results in increasing the stability and reproducibility of the sensor. The relative standard deviation is 1.2 % for determination of 5 nM hydrogen peroxide for 20 times. After the sensor was stored at 4℃ for three months, its change value of the response is lower than 3%. The proposed method has been successfully applied to detect trace hydrogen peroxide in milk sample.

Key words: graphene, gold nanoparticles, functional conducting polymer, biosensor, hydrogen peroxide