Acta Chimica Sinica ›› 1999, Vol. 57 ›› Issue (4): 389-395. Previous Articles     Next Articles

Original Articles

牛血清白蛋白与铬天青S作用机理的研究

马春琪;李克安;赵凤林;童沈阳   

  1. 北京大学化学与分子工程学院
  • 出版日期:1999-04-15 发布日期:1999-04-15

A study on the reaction mechanism between chrome-azurol S and bovine serum albumin

Ma Chunqi;Li Kean;Zhao Fenglin;Tong Shenyang   

  • Online:1999-04-15 Published:1999-04-15

In this paper, three different methods were used to study the binding numbers and binding constants of chrome-azurol S (CAS) on bovine serum albumin (BSA), and almost same results were obtained. It is proposed that the concentration of CAS and pH of the solutions affect the binding mode of CAS and BSA. According to the theory of Forster non-radiative energy transfer, the binding sites of CAS on BSA are found. Taking advantage of fluorescence quenching, the reaction mechanism of CAS and BSA is explored.

Key words: SERUM ALBUMIN, CHROME AZUROLS, REACTION MECHANISM

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