Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (9): 1656-1661. Previous Articles     Next Articles

Original Articles

考马斯亮蓝显色剂变色反应机理的研究

曹稳根;焦庆才;刘茜;陈雷   

  1. 南京大学生命科学学院,南京(210093)
  • 出版日期:2002-09-15 发布日期:2002-09-15

Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250

Cao Wengen;Jiao Qingcai;Liu Qian;Chen Lei   

  1. Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University,Nanjing(210093)
  • Online:2002-09-15 Published:2002-09-15

The mechanism of color changes of Commassie Brilliant Blue G-250 (CBBG) has been investigated by spectrometry. The influence of different experimental conditions on the absorption spectra of CBBG was inspected. It is found that CBBG forms aggregate by hydrophobic interaction in high phosphoric acid concentration. Due to the difference in the aggregative degree, CBBG shows color changes from blue to green to brown in the system.

Key words: Commassie Brilliant Blue G-250, METAGENESIS, HYDROPHOBILITY, REACTION MECHANISM, AGGREGALES

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