Study on mechanism of tribonucleic acid hydrolysis by ribonuclease A using ^3^1P NMR method

Acta Chimica Sinica ›› 1988, Vol. 46 ›› Issue (2): 204-205. Previous Articles

Original Articles

应用^3^1P NMR研究RNase A水解核糖核酸的机制

汪猷;徐耀忠;张伟君

华东师范大学分析测试中心

发布日期:1988-02-15

Study on mechanism of tribonucleic acid hydrolysis by ribonuclease A using ^3^1P NMR method

WANG YOU;XU YAOZHONG;ZHANG WEIJUN

Published:1988-02-15

Abstract

31P NMR spectroscopy was applied to study the reaction course of the hydrolysis of UpU by RNase A. For the 1st time, the individual changes in UpU, U>p (uridine 2',3'-cyclophosphate), and Up (uridine 3'-phosphate) were continuously monitored and quant. determine The 2-step mechanism is thus incontestably proved. The rate of transphosphorylation (k1) is calculated to be ~40.9 s-1, and the rate for the hydrolysis of U>p (k2) is 9.4 s-1.

Key words: RIBONUCLEIC ACID, REACTION MECHANISM, HYDROLYSIS, NUCLEAR MAGNETIC RESONANCE, RIBONUCLEASE

CLC Number:

O653

Cite this article

WANG YOU;XU YAOZHONG;ZHANG WEIJUN. Study on mechanism of tribonucleic acid hydrolysis by ribonuclease A using ^3^1P NMR method[J]. Acta Chimica Sinica, 1988, 46(2): 204-205.

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应用^3^1P NMR研究RNase A水解核糖核酸的机制

Study on mechanism of tribonucleic acid hydrolysis by ribonuclease A using ^3^1P NMR method

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