Chin. J. Org. Chem. ›› 2013, Vol. 33 ›› Issue (10): 2210-2215.DOI: 10.6023/cjoc201305013 Previous Articles     Next Articles

Articles

可逆识别Hg2+的罗丹明基荧光增强型探针及在活细胞成像中的应用

陈稼轩, 田怡, 向清祥, 熊俊如, 张丽群, 李艳   

  1. 乐山师范学院化学学院 乐山 614000
  • 收稿日期:2013-05-10 修回日期:2013-05-31 出版日期:2013-10-25 发布日期:2013-06-09
  • 通讯作者: 田怡 E-mail:tyi3@sina.com
  • 基金资助:

    四川省科技厅应用基础(No. 2009JY0078);四川省教育厅重点(No. 11ZA154)和乐山市科技局重点(No. 2011SZD107)资助项目

A Reversible Fluorescence Enhancement Probe for Hg2+ Based on Rhodamine and Its Application in Live Cell Imaging

Chen Jiaxuan, Tian Yi, Xiang Qingxiang, Xiong Junru, Zhang Liqun, Li Yan   

  1. Department of Chemistry, Leshan Normal University, Leshan 614000
  • Received:2013-05-10 Revised:2013-05-31 Online:2013-10-25 Published:2013-06-09
  • Supported by:

    Project supported by the Science and Technology Department (No. 2009JY0078), the Education Department of Sichuan Province (No. 11ZA154), and the Science and Technology Bureau of Leshan City (No. 2011SZD107).

A novel of Hg2+ fluorescence probe (RS) with rhodamine chromophore was synthesized, and its structure was characterized by 1H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg2+ ions had been investigated in EtOH-HEPES buffer solution (V:V=1:1, pH=7.3) by the ultraviolet spectrophotometry and the fluorescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg2+, the spirolactam ring of probe RS was opened and 1:1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg2+ in L929 living cells demonstrated that the probe RS could be used in biological systems.

Key words: rhodamine B, fluorescent probe, reversibility, Hg2+ ions, living cells