Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (9): 2307-2323.DOI: 10.6023/cjoc201805055 Previous Articles     Next Articles

Special Issue: 合成科学

Reviews

细胞色素P450酶在生物合成及有机合成中的催化功能及其应用

蒋媛媛a,b, 李盛英a,b   

  1. a 中国科学院青岛生物能源与过程研究所 山东省合成生物学省级重点实验室 中国科学院生物燃料重点实验室 青岛 266101;
    b 中国科学院大学 北京 100049
  • 收稿日期:2018-05-30 修回日期:2018-06-29 出版日期:2018-09-25 发布日期:2018-07-24
  • 通讯作者: 李盛英 E-mail:lishengying@qibebt.ac.cn
  • 基金资助:

    山东省自然科学基金重大基础研究(No.ZR2017ZB0207)和国家自然科学基金(Nos.81741115,21472204)资助项目.

Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis

Jiang Yuanyuana,b, Li Shengyinga,b   

  1. a Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101;
    b University of Chinese Academy of Sciences, Beijing 100049
  • Received:2018-05-30 Revised:2018-06-29 Online:2018-09-25 Published:2018-07-24
  • Contact: 10.6023/cjoc201805055 E-mail:lishengying@qibebt.ac.cn
  • Supported by:

    Project supported by the Natural Science Foundation of Shandong Province (No. ZR2017ZB0207) and the National Natural Science Foundation of China (Nos. 81741115, 21472204).

Cytochrome P450 enzymes are widely distributed in nature, which mainly participate in xenobiotics metabolism and natural product biosynthesis. These enzymes are able to recognize various substrates to produce many useful oxidative products through diverse reaction types. P450 enzymes can catalyze selective oxidation of C-H bonds in their substrates under mild conditions. Therefore, a lot of P450 enzymes have been applied in the production of fine chemicals, drugs and chemical intermediates for quite a long time. With the development of protein engineering, redox partner engineering, substrate engineering, metabolic engineering and synthetic biology, it has become possible to obtain the P450 biocatalysts with the desired properties such as high activity, the substrate specificity of interest, and great selectivity to meet the industrial requirements, through rational design and direct evolution of P450 enzymes. Thus, the application scope of P450 enzymes in biosynthesis and organic synthesis has been expanded greatly. The types of reactions that can be catalyzed by P450 enzymes, and the strategies to broaden the reaction scope and to enhance the catalytic efficiency and selectivity are summarized. Finally, the challenges and prospects in the application of cytochrome P450 enzymes in biosynthesis and organic synthesis are discussed.

Key words: cytochrome P450 enzyme, biosynthesis, organic synthesis, protein engineering, substrate engineering, metabolic engineering, synthetic biology, redox partner