Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (2): 339-349.DOI: 10.6023/cjoc201806012 Previous Articles     Next Articles

REVIEW

羰基还原酶在动态动力学拆分中的应用进展

倪国伟a,b, 汤佳伟a, 邹杰a, 陈少欣a, 鞠佃文b, 张福利a   

  1. a 中国医药工业研究总院 上海医药工业研究院 创新药物与制药工艺国家重点实验室 上海 201203;
    b 复旦大学药学院 上海 201203
  • 收稿日期:2018-06-07 修回日期:2018-08-07 出版日期:2019-02-25 发布日期:2018-09-10
  • 通讯作者: 张福利, 鞠佃文 E-mail:zhangfuli1@sinopharm.com;dianwenju@fudan.edu.cn

Recent Advances on Carbonyl Reductases for Dynamic Kinetic Resolution

Ni Guoweia,b, Tang Jiaweia, Zou Jiea, Chen Shaoxina, Ju Dianwenb, Zhang Fulia   

  1. a State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203;
    b School of Pharmacy, Fudan University, Shanghai 201203
  • Received:2018-06-07 Revised:2018-08-07 Online:2019-02-25 Published:2018-09-10
  • Contact: 10.6023/cjoc201806012 E-mail:zhangfuli1@sinopharm.com;dianwenju@fudan.edu.cn

Biocatalysis is a basic method of asymmetric catalysis for preparing chiral Active Pharmaceutical Ingredients, which owns several "green merits":chemo-, regio-and high enantioselectivity. As the development of DNA seqencing, DNA synthesis and protein engineering, suitable enzymes can be efficiently developed for basic researches and industrial applications. Biocatalysis has been keeping as a hot spot in asymmetric synthesis recently. Carbonyl reductases have been widely used for stereoselectively transforming ketones to chiral second alcohols with only one stereocenter. When combining with Dynamic Kinetic Resolution (DKR), the bioreaction with carbonyl reductases can efficiently construct chiral alcohols with two stereocenters in one step. This review highlights the method of its mechanism and nearly twenty examples from research papers and patents for one decade. We attempt to analyze and conclude the characteristics of this method based on chemical structures and enzymes. At last, a practical and developing research method is recommended in three steps:screening-racemization-balance in sequence. It is hoped to be useful for future basic researches and industrial applications.

Key words: asymmetric catalysis, biocatalysis, carbonyl reductases, dynamic kinetic resolution