Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (3): 697-702.DOI: 10.6023/cjoc201808035 Previous Articles     Next Articles

Articles

一种黄酮荧光探针对肼的识别及细胞成像

鞠志宇a, 舒朋华a, 谢智宇a, 蒋雨晴a, 陶伟杰a, 许志红a,b   

  1. a 许昌学院化学化工学院 许昌市化学生物传感与检测重点实验室 许昌 461000;
    b 郑州大学化学与分子工程学院 郑州 450052
  • 收稿日期:2018-08-28 修回日期:2018-11-03 出版日期:2019-03-25 发布日期:2018-11-26
  • 通讯作者: 鞠志宇, 许志红 E-mail:juz1696@163.com;xuzhihong1980@yahoo.com
  • 基金资助:

    河南省科技计划项目(Nos.182102311108,182102310066)、河南省高校科技创新人才计划(No.15HASTIT002)、国家自然科学基金(Nos.21702178,U1304202)资助项目.

A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells

Ju Zhiyua, Shu Penghuaa, Xie Zhiyua, Jiang Yuqinga, Tao Weijiea, Xu Zhihonga,b   

  1. a Xuchang Municipality Key Laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University, Xuchang 461000;
    b College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052
  • Received:2018-08-28 Revised:2018-11-03 Online:2019-03-25 Published:2018-11-26
  • Contact: 10.6023/cjoc201808035 E-mail:juz1696@163.com;xuzhihong1980@yahoo.com
  • Supported by:

    Project supported by the Science and Technology Project of Henan Province (Nos. 182102311108, 182102310066), the Program for Science and Technology Innovation Talents in Universities of Henan Province (No. 15HASTIT002), and the National Natural Science Foundation of China (Nos. 21702178, U1304202).

A 3-hydroxyflavonoid esters HFBA was synthesized by the reaction of 2-hydroxyacetophenone with p-methyl benzaldehyde, and its structure was characterized by NMR and high resolution mass spectrometer. The recognition behaviors of HFBA to hydrazine were investigated and the results show that HFBA exhibits good selectivity and sensitivity to N2H4 with fast response and good anti-interference ability in DMSO/PBS buffer (pH=7.4). Furthermore, the detection limit of HFBA for hydrazine was estimated to be 0.11 μmol/L, and the probe HFBA was successfully applied for the imaging of hydrazine in living cells.

Key words: flavone, fluorescence, hydrazine, biological imaging