Three structural genes which code for the mature small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from tobacco, wheat, and rice, resp., were redesigned with the aid of a computer, totally synthesized by combining chem. methods and enzymic ligation, and expressed in E. coli with high yields. The genes were designed to express the codon usage preferred by the host cell and to include as many unique restriction sites as possible, including EcoRI and XbaI sites at the 5' and 3'-termini of the gene to enable easy transfer from one vector to another.

Key words: WHEAT, PHOSPHATES, ESCHERICHIA COLI, NICOTIANA TABACUM, GENE, GENE EXPRESSION, LOWLAND RICE, RIBOSE