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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (18): 2121-2126. 上一篇    下一篇

研究论文

咖啡因印迹复合膜的制备及性能研究

张朝晖*,a,b 胡宇芳a 张华斌a 李 辉a,b 姚守拙b   

  1. (a吉首大学化学化工学院 吉首 416000)
    (b湖南大学化学传感与计量国家重点实验室 长沙 410082)
  • 投稿日期:2008-10-25 修回日期:2009-02-15 发布日期:2009-12-31
  • 通讯作者: 张朝晖

Preparation and Evaluation of Caffeine Molecularly Imprinted Composite Membrane

Zhang, Zhaohui*,a,b Hu, Yufanga Zhang, Huabina Li, Hui a,b Yao, Shouzhuob   

  1. (a College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000)
    (b State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082)
  • Received:2008-10-25 Revised:2009-02-15 Published:2009-12-31
  • Contact: ZHANG Zhao-Hui

被引次数

17 | 2

以孔径0.45 μm的尼龙微孔滤膜为支撑膜, 咖啡因为模板分子, 丙烯酰胺为功能单体, 乙二醇二甲基丙烯酸酯为交联剂, 采用表面热聚合技术制备对咖啡因具有优良选择透过性的分子印迹复合膜. 采用高效液相色谱技术探讨了不同功能单体印迹聚合膜选择渗透性能的影响, 采用红外光谱分析、X射线衍射分析、热重分析和液相色谱分析等技术对分子印迹膜的化学结构、晶体结构、孔隙率、溶胀性、热稳定性以及选择性等性能进行详细的研究. 结果表明以丙烯酰胺为功能单体的印迹复合膜对咖啡因具有优异的选择透过性, 其分离选择性因子为2.3. 该印迹膜呈现出较好的结合能力, 对印迹分子存在两个结合位点, 最大表观结合量分别为0.146和0.330 μmol/g, 能够较好地应用于山茶叶粗提液中咖啡因的分离和富集, 富集因子为10.52.

关键词: 咖啡因, 印迹膜, 制备, 表征

Employing nylon membrane as the supported membrane, caffeine as template, acrylamide (AA) as functional monomer and ethyleneglycol dimethyacrylate (EGDMA) as cross linker, molecularly imprinted composite membrane was prepared by using a surface thermal-polymerization method. The characterization of the caffeine molecularly imprinted membrane was performed with infrared spectroscopy, X-ray diffraction, thermogravimetric analysis and liquid chromatography techniques in detail. The results show that the imprinted membrane exhibits excellent selectivity for the template with the selectivity factor 2.30 and can be better applied to separate and enrich tea extract with an enrichment factor 10.52. The imprinted membrane exhibited better combination capabilities to the template molecule with two imprinted binding sites. The maximum apparent amounts for higher binding site and lower binding site are 0.146 and 0.330 μmol/g, respectively.

Key words: caffeine, imprinted membrane, preparation, evaluation