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化学学报
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化学学报 ›› 2003, Vol. 61 ›› Issue (5): 755-759. 上一篇    下一篇

研究论文

TaqMan-分子灯标：一种新型的荧光基因检测探针

孔德明;古珑;沈含熙;宓怀风   

  1. 南开大学化学系;南开大学
  • 发布日期:2003-05-15

TaqMan-Molecular Beacon: A New Type of Fluorogenic Probe for Target Gene Detection

Kong Deming;Gu Long;Shen Hanxi;Mi Huaifeng   

  1. Chemical School, Nankai University;State Key Lab of Functional Polymer Materials for Adsorption and Separation, Nankai University
  • Published:2003-05-15

PDF

697

被引次数

7

在TaqMan及分子灯标的基础上开发了一类新型的均相荧光检测探针—— TaqMan-分子灯标(TaqMan-MB)，该探针集合了分子灯标的发夹结构及TaqMan探针降 解作用的工作原理，使检测效果更好．与实时PCR仪联用，可用于靶基因的定量检 测．

关键词: 基因, 荧光, 探针, 聚合酶

A novel homogenous fluorogenic probe, TaqMan-molecular beacon (TaqMan-MB) has been developed from TaqMan and molecular beacons, which combines the hairpin structure of molecular beacons and the cleavage mechanism of TaqMan probes and has much better detection effects than TaqMan or molecular beacons alone. Combing with a real-time PCR instrument, TaqMan-MB presents a promising approach for quantitation of target genes.

Key words: GENE, FLUORESCENCE, PROBES, POLYMERIZATION ENZYME

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