化学学报 ›› 2007, Vol. 65 ›› Issue (13): 1229-1233. 上一篇    下一篇

研究论文

一种新型Zn2+近红外荧光探针的研究

唐友云, 罗春花, 吴朝阳*, 沈国励, 俞汝勤   

  1. (湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082)
  • 投稿日期:2006-12-15 修回日期:2007-01-12 发布日期:2007-07-14
  • 通讯作者: 吴朝阳

A Novel Near-infrared Fluorescent Probe for Zn2+

TANG You-Yun; LUO Chun-Hua; WU Zhao-Yang*; SHEN Guo-Li; YU Ru-Qin   

  1. (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineer-ing, Hunan University, Changsha 410082)
  • Received:2006-12-15 Revised:2007-01-12 Published:2007-07-14
  • Contact: WU Zhao-Yang

Zn2+是多种酶和转录因子的必要成分, 在生物体内起着重要的作用, 检测Zn2+具有十分重要的生物学意义. 我们合成了一种新型近红外荧光探针TAEA-IR-780, 该探针通过配位作用与Zn2+结合后, 荧光显著增强. 通过质谱和氢谱对该探针进行了表征, 并探讨了pH值、其它金属离子对Zn2+检测的影响. 该探针的激发波长为683 nm, 发射波长为750 nm, 可避免对生物活体的损伤, 同时生物体内常见的金属离子对其干扰较小. 该探针对Zn2+的检测下限达1.0× 10-9 mol•L-1, 具有较高的检测灵敏度.

关键词: 近红外, 荧光探针, Zn2+

Zn2+ is an essential component for many enzymes and transcription factors, in which it plays structural or catalytic roles, so its detection is of great biological significance. The development of novel near-infrared fluorescent probe for Zn2+, TAEA-IR-780, was described in this paper, which was obtained by directly attaching an acceptor, tris(2-aminoethyl)amine (TAEA), to a near-infrared fluorophore IR-780. The excitation and emission wavelengths of the TAEA-IR-780 are 683 and 750 nm, respectively, and its fluorescent intensity increases with the addition of Zn2+ under physiological condition (pH=7.4) and the detection limit reaches 1.0×10-9 mol•L-1. Moreover, the fluorescent intensity of TAEA-IR-780 does not increase no-ticeably in the presence of other biologically important cations such as Ca2+ and Mg2+. The results show that the probe can not only effectively minimize cell damage and autofluores-cence by excitation light but also afford sufficient sensitivity and selectivity for the biological detection of Zn2+.

Key words: near-infrared, fluorescent probe, Zn2+