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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (10): 968-974. 上一篇    下一篇

研究论文

新型双核配合物的形成、与DNA的作用机制及荧光性质研究

张黔玲*,1，刘剑洪*,1，任祥忠，张培新1,3，李翠华1, 王芳1，徐宏1，刘建忠2，计亮年2   

  1. (1深圳大学师范学院化学与生物学系 深圳 518060)
    (2中山大学教育部基因工程重点实验室 广州 510275)
    (3西安建筑科技大学材料科学与工程学院 西安 710055)
  • 投稿日期:2005-12-05 修回日期:2006-03-07 发布日期:2006-05-25
  • 通讯作者: 张黔玲

Studies on the Formation, DNA-Binding and Luminescent Properties of New Heterobimetallic Complexes

ZHANG Qian-Ling*,1, LIU Jian-Hong*,1, REN Xiang-Zhong1, ZHANG Pei-Xin1,3, LI Cui-Hua1, WANG Fang1, XU Hong1, LIU Jian-Zhong2, JI Liang-Nian2<   

  1. (1 Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060)
    (2 The Key Laboratory of Gene Engineering of Ministry of Education of China, Zhongshan University, Guangzhou 510275)
    (3 School of Materials Science and Engineering, Xi'an University of Architecture and Technology, Xi'an 710055)
  • Received:2005-12-05 Revised:2006-03-07 Published:2006-05-25
  • Contact: ZHANG Qian-Ling

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被引次数

122 | 14

利用紫外、荧光和粘度等方法研究了含不同配体的钌(II)配合物[Ru(phen)2CImP]2＋(CImP＝3,4-二羟基-咪唑并[4,5-i][1,10]邻菲咯啉)和[Ru(phen)2TPPZ]2＋(TPPZ＝四吡啶[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]吩嗪)与DNA的作用机制, 并研究了配合物与Zn2＋配合后荧光性质变化. 结果表明[Ru(phen)2TPPZ]2＋与DNA以插入模式作用, 而[Ru(phen)2CImP]2＋与DNA则以沟面结合模式作用. 向配合物溶液中滴加Zn2＋后, 配合物[Ru(phen)2TPPZ]2＋和[Ru(phen)2CImP]2＋均可以与Zn2＋形成双核配合物[Ru(phen)2(TPPZ)Zn]4＋和[Ru(phen)2(CImP)Zn]4＋, 配合物的荧光减弱. 与DNA作用后, 配合物仍可以与Zn2＋配位形成双核配合物, 但[Ru(phen)2(TPPZ)Zn]4＋保持插入模式与DNA作用, 配合物的荧光减弱. 而[Ru(phen)2(CImP)Zn]4＋与DNA则由沟面结合改为插入结合, 配合物的荧光增强.

关键词: 多吡啶钌(II)配合物, DNA, Zn2＋, 荧光, 插入结合

The binding modes of polypyridyl ruthenium(II) complexes [Ru(phen)2CImP]2＋(CImP: 3,4-dihydroxyl-imidazo[4,5-i](1,10-phenanthroline) and [Ru(phen)2TPPZ]2＋(TPPZ: tetrapyrido[3,2-c:2', 3'-e:3'',2''-j:2''',3'''-l]phenazine) with calf thymus DNA have been investigated by spectroscopic and viscosity methods. The luminescent properties of coordinated complexes with Zn2＋ were also studied. The experimental results indicated that [Ru(phen)2TPPZ]2＋ bound to DNA by intercalation, while [Ru(phen)2CImP]2＋ bound to DNA by groove binding mode. Both complexes could be coordinated with Zn2＋ to form the heterobimetallic complexes [Ru(phen)2(TPPZ)Zn]4＋ and [Ru(phen)2(CImP)Zn]4＋, and the emission intensity of complexes decreased with increasing the amount of Zn2＋. After binding to DNA, both original complexes could be coordinated with Zn2＋ to form the heterobimetallic complexes, and [Ru(phen)2(TPPZ)Zn]4＋ kept binding to DNA by intercalation, with the emission intensity decreased, while the binding mode of [Ru(phen)2(CImP)Zn]4＋ was changed from groove binding to intercalation, with increase of the emission intensity.

Key words: polypyridyl ruthenium(II) complex, DNA, zinc(II), luminescence, intercalation