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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (15): 1507-1512. 上一篇    下一篇

研究论文

钌(II)多吡啶配合物与DNA相互作用的光切割与荧光性质研究

王海滔1，刘剑洪*,1，张黔玲*,1，胡婷婷1，田生礼2   

  1. (1深圳大学化学与化工学院 深圳市功能高分子重点实验室 深圳 518060)
    (2深圳大学生命科学学院 深圳 518060)
  • 投稿日期:2010-03-04 修回日期:2010-06-28 发布日期:2010-07-21
  • 通讯作者: 王海滔 E-mail:wanght2000cn@yahoo.com.cn
  • 基金资助:

    过渡金属双核配合物的设计合成、荧光性质及与DNA的作用机制研究

DNA-binding Spectrum and Photo-cleavage of Polypyridyl Ruthenium(II) Complex on DNA

Wang Haitao1 Liu Jianhong*,1 Zhang Qianling*,1 Hu Tingting1 Tian Shengli2   

  1. (1 Shenzhen Key Laboratory of Functional Polymer, School of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060)
    (2 School of Life Science, Shenzhen University, Shenzhen 518060)
  • Received:2010-03-04 Revised:2010-06-28 Published:2010-07-21

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利用琼脂糖凝胶电泳法研究了钌(II)多吡啶配位物[Ru(bpy)2(ODCIP)]2＋ (bpy: 2,2 -联吡啶, ODCIP: 含多个配位中心的多吡啶配体, 3,4-二氯基-咪唑并[4,5-f][1,10]邻菲咯啉)对pBR322质粒DNA的光切割作用及其可能机理, 并运用紫外可见吸收光谱、荧光光谱和琼脂糖凝胶电泳法研究了[Ru(bpy)2(ODCIP)]2＋与Zn2＋配位后与DNA的光谱性质和光切割作用. 结果表明[Ru(bpy)2(ODCIP)]2＋对DNA有较好的光切割作用, 其机理可能是产生了超氧阴离子自由基和单线态氧. [Ru(bpy)2(ODCIP)]2＋与Zn2＋配位可能形成的双核配位物[Ru(bpy)2(ODCIP)Zn]4＋与DNA也能进行插入结合, 对DNA的光切割效果并没有明显增强.

关键词: 钌(II)多吡啶配合物, 双核, DNA, 光切割, 荧光

Agarose gel electrophoresis was used to study the binding and photo-cleavage of pBR322 plasmid DNA with polypyridyl ruthenium(II) complex [Ru(bpy)2(ODCIP)]2＋ (bpy: 2,2 -dipyridyl, ODCIP: 3,4- dichloro-imidazo[4,5-f][1,10]phenanthroline). Since the polypyridyl ligand ODCIP has multi coordination sites, the coordination of [Ru(bpy)2(ODCIP)]2＋ with Zn2＋ and their binding and photo-cleavage effect on DNA were also investigated by UV-vis absorption spectroscopy, fluorescent spectroscopy and agarose gel electrophoresis experiments. The results showed that the complex had a good photo-cleavage effect and might produce a combination of superoxide anion radicals and singlet oxygen mechanism for the DNA scission. The binuclear complex [Ru(bpy)2(ODCIP)Zn]4＋ could be formed by [Ru(bpy)2(ODCIP)]2＋ and Zn2＋, and it bound to DNA by intercalation but the photo-cleavage of pBR322 plasmid DNA had not been notably enhanced.

Key words: polypyridyl ruthenium(II) complex, binuclear, DNA, photo-cleavage, luminescence