化学学报 ›› 2012, Vol. 0 ›› Issue (05): 617-623 .DOI: 10.6023/A1107302 上一篇    下一篇

研究论文

有序中孔炭/四氧化三铁复合材料的制备及其用于血红蛋白的电化学研究

郑静a,b, 赵家昌a, 龚晨a, 刘萍a, 陈瑜a, 黄焕a, 何品刚b, 方禹之b   

  1. a 上海工程技术大学化学化工学院 上海 201620;
    b 华东师范大学化学系 上海 200062
  • 投稿日期:2011-07-30 修回日期:2012-01-12 发布日期:2012-03-17
  • 通讯作者: 郑静 E-mail:kkzhengjing707@163.com
  • 基金资助:

    国家自然科学基金(No. 20675031)和上海市教委(No. 09YZ374)资助项目.

Preparation of the Ordered Mesoporous Carbon/Fe3O4 and Its Application for the Direct Electrochemistry of Hemoglobin

Zheng Jinga,b, Zhao Jiachanga, Gong Chena, Liu Pinga, Chen Yua, Huang Huana, He Pingangb, Fang Yuzhib   

  1. a College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620;
    b Department of Chemistry, East China Normal University, Shanghai 200062
  • Received:2011-07-30 Revised:2012-01-12 Published:2012-03-17
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 20675031) and Innovation Program of Shanghai Municipal Education Commission (No. 09YZ374).

采用微湿含浸法制备了有序中孔炭/四氧化三铁磁性材料. 采用透射电镜和X 射线衍射对复合材料进行了表征.将血红蛋白(Hb)固定于材料表面, 对其直接电化学行为进行了研究, 结果表明Hb 在该材料内仍保持了其生物活性, 在pH=7.0 的PBS 缓冲液中, 血红蛋白表现出一对峰形良好的准可逆氧化还原峰, 为Hb 的Fe(III)/Fe(II)电对的特征峰, 求出式电位E0'为-0.306 V, 电子转移数为n=1.226, 电荷传递系数为α=0.51, 表观异相电子转移速率常数为KS=0.0144s-1. 在3.00×10-6 到1.50×10-4 mol/L 浓度范围内, 血红蛋白的浓度与其响应电流呈良好的线性关系, 线性相关系数为R=0.9924, 最低检测限为0.270×10-6 mol/L.

关键词: 有序中孔炭, 四氧化三铁, 制备, 血红蛋白, 直接电化学

The novel magnetic composites of ordered mesoporous carbon/Fe3O4 were prepared with a micro-wetness impregnation method. The composites were characterized by transmission electron microscope and X-ray diffraction. Hemoglobin was immobilized onto the surface of the composites, and was shown to keep its biological activity well. The electrochemical behavior of Hb was studied, and a pair of well-defined, quasi-reversible cyclic voltammetric redox peaks was obtained, which was characteristics of Hb Fe(III)/Fe(II) redox couples. The formal potential (E0'), the electron transfer number (n), and transfer coefficient (α) were calculated to be -0.306 V, 1.226 and 0.51, respectively. The average apparent heterogeneous electron transfer rate constant (KS) obtained for Hb was 0.0144 s-1. Linear sweep voltammetric measurement of Hb indicated that the peak currents of Hb were linear to its concentration ranging from 3.00×10-6 to 1.50×10-4 mol/L with the detection limits of 0.270×10-6 mol/L.

Key words: ordered mesoporous carbon, ferriferrous oxide, preparation, hemoglobin, direct electrochemistry