Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250

Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (9): 1656-1661. Previous Articles Next Articles

Original Articles

考马斯亮蓝显色剂变色反应机理的研究

曹稳根;焦庆才;刘茜;陈雷

南京大学生命科学学院,南京(210093)

发布日期:2002-09-15

Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250

Cao Wengen;Jiao Qingcai;Liu Qian;Chen Lei

Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University,Nanjing(210093)

Published:2002-09-15

Abstract

The mechanism of color changes of Commassie Brilliant Blue G-250 (CBBG) has been investigated by spectrometry. The influence of different experimental conditions on the absorption spectra of CBBG was inspected. It is found that CBBG forms aggregate by hydrophobic interaction in high phosphoric acid concentration. Due to the difference in the aggregative degree, CBBG shows color changes from blue to green to brown in the system.

Key words: Commassie Brilliant Blue G-250, METAGENESIS, HYDROPHOBILITY, REACTION MECHANISM, AGGREGALES

CLC Number:

Cite this article

Cao Wengen;Jiao Qingcai;Liu Qian;Chen Lei. Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250[J]. Acta Chimica Sinica, 2002, 60(9): 1656-1661.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Guanglong Huang, Xiao-Song Xue. Computational Study on the Mechanism of Chen’s Reagent as Trifluoromethyl Source [J]. Acta Chimica Sinica, 2024, 82(2): 132-137.
[2]	Guoqing Cui, Yiyang Hu, Yingjie Lou, Mingxia Zhou, Yuming Li, Yajun Wang, Guiyuan Jiang, Chunming Xu. Research Progress on the Design, Preparation and Properties of Catalysts for CO₂ Hydrogenation to Alcohols [J]. Acta Chimica Sinica, 2023, 81(8): 1081-1100.
[3]	Xinpu Fu, Xiuling Wang, Weiwei Wang, Rui Si, Chunjiang Jia. Fabrication and Mechanism Study of Clustered Au/CeO₂ Catalyst for the CO Oxidation Reaction^★ [J]. Acta Chimica Sinica, 2023, 81(8): 874-883.
[4]	Luocong Wang, Zhewei Li, Caiwei Yue, Peihuan Zhang, Ming Lei, Min Pu. Theoretical Study on the Isomerization Mechanism of Azobenzene Derivatives under Electric Field [J]. Acta Chimica Sinica, 2022, 80(6): 781-787.
[5]	Siming Yang, Airong Liu, Jing Liu, Zhaoli Liu, Weixian Zhang. Advance of Sulfidated Nanoscale Zero-Valent Iron: Synthesis, Properties and Environmental Application [J]. Acta Chimica Sinica, 2022, 80(11): 1536-1554.
[6]	Yilong Hua, Donghan Li, Tianhang Gu, Wei Wang, Ruofan Li, Jianping Yang, Wei-xian Zhang. Enrichment of Uranium from Aqueous Solutions with Nanoscale Zero-valent Iron: Surface Chemistry and Application Prospect [J]. Acta Chimica Sinica, 2021, 79(8): 1008-1022.
[7]	Huang Rongyi, Shen Qiong, Zhang Chao, Zhang Shaoyong, Xu Heng. Studies on the Mechanism of the Transition Metal-Catalyzed Reaction of Organonitrile with Sodium Azide [J]. Acta Chimica Sinica, 2020, 78(6): 565-571.
[8]	Wu Weirong, Yuan Xiaomin, Hou Hua, Wang Baoshan. Theoretical Investigations on the Mechanisms for the Reactions of Sevoflurane Radicals[(CF₃)₂C(·)OCH₂F, (CF₃)₂CHOC(·)HF] with O₂and the OH· Radicals Regeneration [J]. Acta Chim. Sinica, 2018, 76(10): 793-801.
[9]	Mu Weihua, Ma Yao, Fang Decai, Wang Rong, Zhang Haina. Computational Insights into the Diels-Alder-alike Reactions of 1-Iodo-2-Lithio-o-Carborane with Fulvenes [J]. Acta Chim. Sinica, 2018, 76(1): 55-61.
[10]	Huang Xiao-yue, Wang Wei, Ling Lan, Zhang Wei-xian. Heavy Metal-nZVI Reactions: the Core-shell Structure and Applications for Heavy Metal Treatment [J]. Acta Chim. Sinica, 2017, 75(6): 529-537.
[11]	Tang Jing, Tang Lin, Feng Haopeng, Dong Haoran, Zhang Yi, Liu Sishi, Zeng Guangming. Research Progress of Aqueous Pollutants Removal by Sulfidated Nanoscale Zero-valent Iron [J]. Acta Chim. Sinica, 2017, 75(6): 575-582.
[12]	Ding Shuang, Ge Qingfeng, Zhu Xinli. Research Progress in Ketonization of Biomass-derived Carboxylic Acids over Metal Oxides [J]. Acta Chim. Sinica, 2017, 75(5): 439-447.
[13]	Yang Zhen, Xue Yijiang, He Yuanhang. Thermal Sensitivity of CL20/DNB Co-crystal Research via Molecular Dynamics Simulations [J]. Acta Chim. Sinica, 2016, 74(7): 612-619.
[14]	Ma Jun, Li Rong, Ren Kuiwei, Ma Xilong, Zhu Kaili, Geng Zhiyuan. Mechanism and Spin-orbit Coupling Study for Two State Reaction Ni₂⁺ with Cyclohexane [J]. Acta Chim. Sinica, 2015, 73(5): 431-440.
[15]	Wang Ying, Zhang Limin, Hu Tianjun. Progress in Oxygen Reduction Reaction Electrocatalysts for Metal-Air Batteries [J]. Acta Chim. Sinica, 2015, 73(4): 316-325.

考马斯亮蓝显色剂变色反应机理的研究

Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments