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Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (9): 1101-1106. Previous Articles Next Articles
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许子竞,林翠梧*
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国家自然科学基金项目
XU Zi-Jing, LIN Cui-Wu
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A polysaccharide (BRP) was obtained from Blumea riparia, by hot water extraction, deproteination using Sevag method, precipitation with ethanol, and fractionation through DEAE-cellulose column, Sephadex G-200 and Sepharose 6 F.F. Gel filtration chromatography. Based on UV and IR spectrum, HPLC, HPGPC and GC-MS chromatography, NMR (1H NMR, 13C NMR, HMQC, HMBC) technology and methylation reaction, the structure of BRP was identified. The results showed that BRP was hightly purified with average molecular weight of 3.3×104 Da determinted by HPGPC. And it was composed of Fru by HPLC analysis. The backbone of BRP had a consisting of 1,2-linked β-D-fructofuranosyl residues. Finally, the structure of BRP was fixed on as: β-D-Fruf-(2[→1)-β-D-Fruf(2→]n-1)-β-D-Fruf.
Key words: polysaccharide (BRP), isolation, NMR, methylation, structure characterization
XU Zi-Jing, LIN Cui-Wu. Structure Identification of Polysaccharide BRP from Blumea Riparia[J]. Acta Chimica Sinica, 2011, 69(9): 1101-1106.
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