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研究论文

杏香兔耳风内生真菌Penicillium sp. L02次级代谢产物与生物活性研究

刘丽†,a, 毕晓宾†,b, 关鑫磊c,d, 吴涛c,d, 郭志磊e, 宋红萍c,d, 朱小强c,d,*, 雷亮c,d,*   

  1. a武汉市第四医院 中医科,湖北武汉 430033;
    b华中科技大学 药学院,湖北武汉 430030;
    c武汉市第四医院,湖北省运动医学中心,湖北省骨科临床医学研究中心,运动损伤与精准治疗湖北省重点实验室,湖北武汉 430033;
    d武汉市第四医院,中心实验室,湖北武汉 430033;
    e武汉市第四医院,药学部,湖北武汉 430033
  • 收稿日期:2026-05-25 修回日期:2026-06-05
  • 基金资助:
    湖北省自然科学基金联合基金项目（No. 2026AFC1312）,湖北省自然科学基金面上项目（No. 2023AFB1057）,国家自然科学基金青年基金项目（No. 32000282）

Study on Secondary Metabolites and their Biological Activities from an Endophytic Fungus Penicillium sp. L02 of Ainsliaea fragrans

Liu Li†,a, Bi Xiaobin†,b, Guan Xinleic,d, Wu Taoc,d, Guo Zhileie, Song Hongpingc,d, Zhu Xiaoqiangc,d,*, Lei Liangc,d,*   

  1. aDepartment of Traditional Chinese Medicine, Wuhan Fourth Hospital, Wuhan, 430033;
    bSchool of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030;
    cHubei Provincial Sports Medicine Center, Hubei Provincial Clinical Research Center for Orthopaedics, Hubei Key Laboratory of Sports Injury and Precision Therapy, Wuhan Fourth Hospital, Wuhan, 430033;
    dCentral Laboratory, Wuhan Fourth Hospital, Wuhan, 430033;
    eDeportment of Pharmacy, Wuhan Fourth Hospital, Wuhan, 430033
  • Received:2026-05-25 Revised:2026-06-05
  • Contact: *E-mail: zhuxiaoqiang1992@126.com; leiliang@hust.edu.cn
  • About author:† These authors contributed equally to the study
  • Supported by:
    Project supported by Hubei Provincial Natural Science Foundation-Joint Fund Project (No. 2026AFC1312); Hubei Provincial Natural Science Foundation- General Project (No. 2023AFB1057); Natural Science Foundation of China (No. 32000282)

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补充材料

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本研究对1株传统中药杏香兔耳风来源真菌Penicillium sp. L02的次级代谢产物进行研究,从中分离得到1个新的andrastin型杂萜化合物atlantinone C（1）和4个类似物（2~5）。通过1D、2D核磁共振波谱、高分辨质谱和X-ray单晶衍射确定了新化合物1的平面结构和绝对构型。体外抗炎结果显示,化合物4和5对LPS诱导的RAW 264.细胞的NO释放具有显著的抑制活性,IC50值分别为29.09 ± 1.33 μM和26.71 ± 1.40 μM,强于阳性药吲哚美辛（36.69 ± 1.23 μM）。体外抗4种临床常见耐药菌活性测试表明,化合物1~5的最低抑菌浓度（MIC）均大于64.0 μg/mL。

关键词: 杏香兔耳风, Penicillium sp. L02, 内生真菌, 次生代谢产物, 结构鉴定, 抗炎活性, 抗菌活性

A new andrastin-type meroterpenoids derivative, atlantinone C (1) and four known analogues, including bialorastin C (2),peniciterpenolide A (3),peniandrastin G (4),10-formyl andrastone A (5) were isolated from an endophytic fungus Penicillium sp. L02 in this study. Their structures were elucidated by detailed analysis of comprehensive spectroscopic data, and the structure of 1 was further determined by X-ray diffraction analysis. In vitro anti-inflammatory assays revealed that compounds 4 and 5 exhibited significant inhibitory activities against NO production in LPS-induced RAW 264.7 cells, with IC50 values of 29.09 ± 1.33 μM and 26.71 ± 1.40 μM, respectively, which were more potent than the positive control indomethacin (36.69 ± 1.23 μM). In vitro antibacterial evaluation against four common clinical drug-resistant bacteria indicated that the MIC of compounds (1-5) were greater than 64.0 μg/mL, showing no clinically significant antibacterial activity.

Key words: Ainsliaea fragrans Champ., Penicillium sp. L02, endophytic fungus, secondary metabolites, structural identification, anti-inflammatory activity, antibacterial activity