天山假狼毒根部来源曲霉Aspergillus sp. L06的次级代谢产物及抗菌活性研究

doi:10.6023/cjoc202511024

研究简报

天山假狼毒根部来源曲霉Aspergillus sp. L06的次级代谢产物及抗菌活性研究

董莉萍^†,a, 杨金花^†,a, 方容^a, 张兴^a, 朱小强^a, 毕晓宾^b,*, 雷亮^a,*

^a武汉市第四医院，湖北武汉 430033;
^b华中科技大学药学院，湖北武汉 430030

收稿日期:2026-02-03 修回日期:2026-03-02
作者简介:^† 共同第一作者
基金资助:
武汉市卫生健康委员会科研项目(Nos. WZ22Q21 & WZ24B14)资助项目; 国家自然科学基金（No. 32000282）.

Study on the Secondary Metabolites and antibacterial activity of a fungus Aspergillus sp. L06 from the roots of Stelleropsis tianschanica (Pobed)

Li-Ping Dong^†,a, Jin-Hua Yang^†,a, Rong Fang^a, Xing Zhang^a, Xiao-Qiang Zhu^a, Xiao-Bin Bi^b,*, Liang Lei^a,*

^aWuhan Fourth Hospital, Wuhan, 430033;
^bSchool of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030

Received:2026-02-03 Revised:2026-03-02
Contact: *E-mail: leiliang@hust.edu.cn; bxbin@hust.edu.cn
About author:^†(These authors contributed equally to this work)
Supported by:
Project supported by the Wuhan Municipal Health Commission (Nos. WZ22Q21 & WZ24B14); the National Natural Science Foundation of China (Nos. 32000282 & 823041523).

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摘要/Abstract

本研究采用正相硅胶、反相硅胶、凝胶柱色谱以及高效液相色谱等方法，对1株天山假狼毒根部来源真菌Aspergillus sp. L06的大米发酵产物进行分离纯化，得到4个化合物。通过理化性质和波谱方法分别鉴定为aspeverchromone A（1）、杂色曲霉素（2）、nidurufin（3）、versicolorin B（4），其中化合物1为新化合物。体外抗菌活性结果表明化合物1对多重耐药铜绿假单胞菌较强抑制作用，最小抑菌浓度为4.0 μg/mL，优于阳性对照庆大霉素。

关键词: 天山假狼毒, Aspergillus sp. L06, 次级代谢产物, 结构鉴定, 体外抗菌活性

This study aims to find monomer compounds with antimicrobial activity from the secondary metabolites of a soil-derived fungus Aspergillus sp. L06 isolated from the roots of Stelleropsis tianschanica (Pobed) in Xinjiang pastoral areas. Four compounds were obtained from the ethyl acetate layer of the fermentation of Aspergillus sp. L06 by silica gels, Sephadex LH-20, ODS column chromatography, and HPLC methods. Their structures were identified as aspeverchromone A (1), sterigmatocystin (2), nidurufin (3), versicolorin B (4) by NMR, MS and other spectroscopic techniques. Among them, compound 1 is a new compound. The results of in vitro antibacterial activity indicated that compound 1 exhibited a strong inhibitory effect on multidrug-resistant Pseudomonas aeruginosa, with a minimum inhibitory concentration of 4.0 μg/mL, which was superior to the positive control gentamicin.

Key words: Stelleropsis tianschanica (Pobed), Aspergillus sp. L06, secondary metabolites, structural identification, in vitro antibacterial activity

引用此文

董莉萍, 杨金花, 方容, 张兴, 朱小强, 毕晓宾, 雷亮. 天山假狼毒根部来源曲霉Aspergillus sp. L06的次级代谢产物及抗菌活性研究[J]. 有机化学, doi: 10.6023/cjoc202511024.

Li-Ping Dong, Jin-Hua Yang, Rong Fang, Xing Zhang, Xiao-Qiang Zhu, Xiao-Bin Bi, Liang Lei. Study on the Secondary Metabolites and antibacterial activity of a fungus Aspergillus sp. L06 from the roots of Stelleropsis tianschanica (Pobed)[J]. Chinese Journal of Organic Chemistry, doi: 10.6023/cjoc202511024.

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