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有机化学
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有机化学 ›› 1993, Vol. 13 ›› Issue (3): 309-313. 上一篇    下一篇

研究论文

烟草、小麦和水稻三个设计的rbcs结构基因的全合成和在大肠肝菌中的表达

陈海宝   

  1. 中国科学院上海有机化学研究所
  • 发布日期:1993-06-25

Total synthesis and expressions in E.coli of three redesigned genes for the mature small subunit (rbcs)of rubisco from tobacco wheat and rice

CHEN HAIBAO   

  • Published:1993-06-25

用计算机辅助再设计了烟草、小麦和水稻的核糖酮-1,5-双磷酸羧化酶/氧化酶的成熟亚基(rbcS)的三个基因,结合化学方法和酶促配位进行了全合成,并在大肠杆菌中以高得产表达.

关键词: 磷酸酯, 基因, 大肠杆菌, 小麦, 羧酸酶, 烟草, 基因表达, 核糖, 水稻

Three structural genes which code for the mature small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from tobacco, wheat, and rice, resp., were redesigned with the aid of a computer, totally synthesized by combining chem. methods and enzymic ligation, and expressed in E. coli with high yields. The genes were designed to express the codon usage preferred by the host cell and to include as many unique restriction sites as possible, including EcoRI and XbaI sites at the 5' and 3'-termini of the gene to enable easy transfer from one vector to another.

Key words: WHEAT, PHOSPHATES, ESCHERICHIA COLI, NICOTIANA TABACUM, GENE, GENE EXPRESSION, LOWLAND RICE, RIBOSE

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