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有机化学
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有机化学 ›› 1995, Vol. 15 ›› Issue (1): 72-75. 上一篇    下一篇

研究论文

诱导根癌农杆菌vir区基因表达水稻代谢物的分离,鉴定和生物活性研究

刘煜;黄志纾;肖剑国;古练权;许东晖;李宝健;吴厚铭;马侃   

  1. 中山大学化学系;中山大学生物工程研究中心;中国科学院上海有机化学所生命有机化学国家
  • 发布日期:1995-02-25

The purification , indentification and biological activity of metabolites capable of inducing Agrobacterium vir gene expression isolated from rice (Oryza sative L.)

LIU YU;HUANG ZHISHU;XIAO JIANGUO;GU LIANQUAN;XU DONGHUI;LI BAOJIAN;WU HOUMING;MA KAN   

  • Published:1995-02-25

被引次数

14

从孕穗期至开花期水稻(oryza sative L. cv. lndia IR 72)的茎叶提取液中, 筛选并分离出二种能高效诱导根癌农杆菌(Agrobacterium tumfaciens) vir 区基因表达的信号分子,这两种信号分子的化学结构分别是5,7,4'-三羟基-3'-5'-二甲氧基黄酮和5,4'-二羟基-3',5'-二甲氧基-7-β-D-葡萄糖氧基黄酮.

关键词: 广东省青年科学基金, 分离, 水稻, 黄酮类化合物, 诱导物, 农杆菌VIR区, 鉴定, 基因

Two flavones are isclated from leaf and stem extracts of panicle-differentiating to flowering rice (Oryza sative L. ) plants. Based on the spectroscopy analysis, the structures of these two compounds are identified as 5,7,4'-trihydroxy- 3',5'-dimethoxy-foavone (A) and 5,4'-dihydroxy-3',5'- dimethoxy-7-β-D-glucosyloxy-foavone (B), respectively. These two compounds were able to strongly induce vir gene expression of Agrobacterium tumefaciens.

Key words: SEPARATION, FLAVONOID, LOWLAND RICE, IDENTIFICATION, INDUCER, GENE

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