有机化学 ›› 2024, Vol. 44 ›› Issue (7): 2377-2380.DOI: 10.6023/cjoc202401038 上一篇    下一篇

研究简报

江西嗜酸链霉菌去铁胺E及金属络合物的分离鉴定与生物活性研究

李金达a,b, 程伯涛b, 黄积武b,*(), 刘文b,*()   

  1. a 上海师范大学化学与材料科学学院 上海 200233
    b 中国科学院上海有机化学研究所 生命过程小分子调控全国重点实验室 上海 200032
  • 收稿日期:2024-01-30 修回日期:2024-03-11 发布日期:2024-04-09
  • 基金资助:
    科技部重大专项(2022YFC2303100); 国家自然科学基金(22193070); 国家自然科学基金(32030002)

Study on Isolation, Identification and Bioactivity of Desferrioxamine E and Its Metal-Complexes from Streptacidiphilus jiangxiensis

Jinda Lia,b, Botao Chengb, Jiwu Huangb(), Wen Liub()   

  1. a College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200233
    b State Key Labratory of Chemical Biology, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
  • Received:2024-01-30 Revised:2024-03-11 Published:2024-04-09
  • Contact: E-mail: huangjiwu@sioc.ac.cn;wliu@sioc.ac.cn
  • Supported by:
    National Key Research and Development Program of China(2022YFC2303100); National Natural Science Foundation of China(22193070); National Natural Science Foundation of China(32030002)

嗜酸链霉菌属细菌的研究主要集中在新物种的分离和鉴定上, 而其次级代谢产物的研究则相对较少. 从江西嗜酸链霉菌的发酵液中分离得到一个新的铝配合物铝草铵(alumioxamine, 3)和两个已知化合物去铁胺E (desferrioxamine E, 1)和铁草铵E (ferrioxamine E, 2), 并通过MS、IR、NMR以及单晶X射线衍射技术对其结构进行鉴定. 从结构上来看, 化合物3以铝为中心, 与配体的六个O原子相连, 形成六齿配合物. 此外, 体外细胞毒活性实验表明, 在100 μmol/L浓度时化合物1对4种人肿瘤细胞(Hela、PANC1、A375和MHCC-97H)均具有一定的抑制活性, 细胞存活率为4.8%~66.4%.

关键词: 江西嗜酸链霉菌, 次级代谢产物, 配合物, 细胞毒活性, 晶体结构

Research on the genus Streptacidiphilus was mainly focused on the isolation and identification of new species, with relatively less emphasis on the study of secondary metabolites. A new aluminium-complex alumioxamine (3), and two known compounds desferrioxamine E (1) and ferrioxamine E (2) were isolated from the fermentation broth of Streptacidiphilus jiangxiensis CGMCC 4.1857. Their structures were elucidated by MS, IR, NMR and single crystal X-ray diffraction technologies. Structurally, compound 3 features aluminum(III) as the central atom, which is bonded to six oxygen atoms of the ligand, forming a hexadentate complex. Furthermore, in vitro cytotoxicity assays showed that compound 1 exhibited potential inhibitory activity against four human tumor cell lines (Hela, PANC1, A375 and MHCC-97H) at a concentration of 100 μmol/L with cell viability ranging from 4.8% to 66.4%.

Key words: Streptacidiphilus jiangxiensis, secondary metabolites, complexes, cytotoxic activity, crystal structure