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研究论文

Streptomycessp.N12W1565中抑菌溴代naphthacemycin的表征

彭淑娅a,b, 路新华c, 花强*,a, 唐功利*,b   

  1. a华东理工大学生物工程学院 上海 200030;
    b中国科学院上海有机化学研究所 上海 200032;
    c微生物药物国家工程研究中心 石家庄 050015
  • 收稿日期:2025-12-25 修回日期:2026-01-13
  • 基金资助:
    国家自然科学基金(No. 22137009)资助项目.

Characterization of Antibacterial Bromo-naphthacemycin from Streptomyces sp. N12W1565

Peng Shu-Yaa,b, Lu Xin-Huac, Hua Qiang*,a, Tang Gong-Li*,b   

  1. aDepartment of Applied Biology, East China University of Science and Technology, Shanghai 200030;
    bShanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032;
    cNational Microbial Medicine Engineering and Research Center, Shijiazhuang 050015
  • Received:2025-12-25 Revised:2026-01-13
  • Contact: *E-mail: gltang@sioc.ac.cn; qhua@ecust.edu.cn
  • Supported by:
    National Natural Science Foundation of China (No. 22137009).

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卤化天然产物naphthacemycin分离于中草药千里光茎上的一株链霉菌Streptomyces sp. N12W1565,前期已确定该天然产物的多氯取代反应由naphthacemycin生物合成基因簇中的多位点氯化酶NatC催化,通过调控该链霉菌生长环境中的卤素原子种类有望实现不同卤化天然产物的生成。本研究通过优化该链霉菌的发酵培养条件实现溴代naphthacemycin的产生,并利用高分辨电喷雾电离质谱和核磁共振成功表征三个新的一溴取代naphthacemycin,其中化合物5和7表现出显著优于未发生溴化的化合物1的抑菌活性。

关键词: II型聚酮类天然产物, naphthacemycin, 卤化, 抑菌

The halogenated natural product naphthacemycin was isolated from Streptomyces sp. N12W1565 strain associated with the stems of the traditional Chinese medicinal herb Senecio scandens Buch.-Ham. Ex D. Don. Previous studies have established that polychlorination of this metabolite was catalyzed by the multi-site halogenase NatC encoded within the naphthacemycin biosynthetic gene cluster, suggesting that modulation of halide species in the culture medium can enable the generation of diverse halogenated analogues. In this study, brominated naphthacemycins were produced by optimizing the fermentation conditions of this strain. Three novel monobrominated naphthacemycins were successfully characterized by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy, among which compound 5 and 7 exhibited significantly enhanced antibacterial activity compared to the non-brominated compound 1.

Key words: type II polyketides natural product, naphthacemycin, halogenation, antibacterial