Chin. J. Org. Chem. ›› 2017, Vol. 37 ›› Issue (2): 423-428.DOI: 10.6023/cjoc201608003 Previous Articles     Next Articles

ARTICLE

一种苯并噻唑衍生物对H2S的荧光识别及细胞成像

何平a, 汤立军a, 钟克利a, 侯淑华a, 燕小梅b   

  1. a 渤海大学化学化工学院 锦州 121013;
    b 大连医科大学检验医学院 大连 116044
  • 收稿日期:2016-08-04 修回日期:2016-09-19 发布日期:2016-10-11
  • 通讯作者: 汤立军, 燕小梅 E-mail:ljtang@bhu.edu.cn;xmyan1978@sina.com
  • 基金资助:

    国家自然科学基金(Nos.21476029,U1608222)及辽宁省高等学校优秀人才支持计划(No.LR2015001)资助项目.

Fluorescence Recognition of H2S by a Benzothiazole Derivative and Its Live Cell Imaging

He Pinga, Tang Lijuna, Zhong Kelia, Hou Shuhuaa, Yan Xiaomeib   

  1. a College of Chemistry and Chemical Engineering, Bohai University, Jinzhou 121013;
    b College of Laboratory Medicine, Dalian Medical University, Dalian 116044
  • Received:2016-08-04 Revised:2016-09-19 Published:2016-10-11
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 21476029, U1608222) and the Liaoning Excellent Talents in University (No. LR2015001).

A new benzothiazole-derived fluorescence probe 2-(4-azidobenzyl)oxy-3-(benzo[d]thiazol-2-yl)-5-methylben-zaldehyde (HBTA) was synthesized from 3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methylbenzaldehyde, and the structure of HBTA was characterized. The recognition behaviors of HBTA to H2S were investigated and the results show that HBTA exhibits good selectivity and sensitivity to H2S with fast response and good anti-interference ability. The probe can be applied to detect H2S in a broad pH range, and the detection limit of HBTA for H2S was estimated to be 9.09×10-7 mol·L-1. Cell imaging studies reveal that HBTA is capable of detect H2S in live cells.

Key words: hydrogen sulfide, fluorescent probe, benzothiazole, cells imaging