“Turn-On” Fluorescent Probe for Hypochlorite: Successful Bioimaging and Real Application in Tap Water

doi:10.6023/cjoc202003034

Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (7): 1941-1947.DOI: 10.6023/cjoc202003034 Previous Articles Next Articles

荧光增强型次氯酸根传感器及其在细胞成像与自来水检测中的应用

程晓红^a, 李爽^b, 汪竞阳^a, 李望南^a

a 湖北文理学院低维光电材料与器件湖北省重点实验室湖北襄阳 441053;
b 湖北航天化学技术研究所湖北襄阳 441003

收稿日期:2020-03-13 修回日期:2020-04-12 发布日期:2020-05-11
通讯作者: 程晓红 E-mail:chengxiaohong0807@126.com
基金资助:
湖北省自然科学基金（No.2018CFB454）、湖北文理学院学科开放基金（No.XK2020042）、湖北文理学院教师科研能力培育基金（No.2020kypyfy031）资助项目.

“Turn-On” Fluorescent Probe for Hypochlorite: Successful Bioimaging and Real Application in Tap Water

Cheng Xiaohong^a, Li Shuang^b, Wang Jingyang^a, Li Wangnan^a

a Hubei Key Laboratory of Low Dimensional Optoelectronic Materials and Devices, Hubei University of Arts and Science, Xiangyang, Hubei 441053;
b Hubei Institute of Aerospace Chemical Technology, Xiangyang, Hubei 441003

Received:2020-03-13 Revised:2020-04-12 Published:2020-05-11
Supported by:
Project supported by the Natural Science Foundation of Hubei Province (No. 2018CFB454), the Project of Hubei University of Arts and Science (No. XK2020042) and the Teachers' Scientific Ability Cultivation Foundation of Hubei University of Arts and Science (No. 2020kypyfy031).

1. 201941S.pdf(1033KB)

Abstract

Taking advantage of the special oxidation property of hypochlorite, two novel coumarin-type fluorescent probes C1 and C2 were synthesized for ClO^- detection. Both probes could detect ClO^- anions in aqueous solution efficiently with rapid switching-on fluorescent methods. Especially, probe C2 displayed dramatic enhancement in fluorescence emission spectra with the detection limit of 1.8×10^-7 mol/L. In addition to its high selectivity for ClO^- rather than other common anions and reactive oxygen species, C2 was successfully applied to the bioimaging in HeLa cells with ‘turn-on’ fluorescent methods. Moreover, probe C2 could be used for the analysis of ClO^- levels in tap water and potentially in environmental samples.

Key words: fluorescent probe, hypochlorite, good solubility, bioimaging application

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Cheng Xiaohong, Li Shuang, Wang Jingyang, Li Wangnan. “Turn-On” Fluorescent Probe for Hypochlorite: Successful Bioimaging and Real Application in Tap Water[J]. Chinese Journal of Organic Chemistry, 2020, 40(7): 1941-1947.

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荧光增强型次氯酸根传感器及其在细胞成像与自来水检测中的应用

“Turn-On” Fluorescent Probe for Hypochlorite: Successful Bioimaging and Real Application in Tap Water

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