Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51

doi:10.6023/cjoc201708041

Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (1): 246-252.DOI: 10.6023/cjoc201708041 Previous Articles Next Articles

Special Issue: 庆祝吴养洁院士九十华诞专辑

Notes

类乳腺癌易感基因BRCA1肽的设计、合成及与抑癌基因蛋白RAD51肽段的相互作用

李林璐^a, 吕名秀^a,b, 卢奎^b,c, 刘广斌^a, 彭露^b

a 郑州大学化学与分子工程学院郑州 450001;
b 河南工程学院材料与化学工程学院郑州 450007;
c 郑州工程技术学院化工食品学院郑州 450044

收稿日期:2017-08-21 修回日期:2017-10-26 发布日期:2017-10-31
通讯作者: 卢奎 E-mail:luckyluke@haue.edu.cn
基金资助:
国家自然科学基金（Nos.21572046，21172054）资助项目.

Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51

Li Linlu^a, Lü Mingxiu^a,b, Lu Kui^b,c, Liu Guangbin^a, Peng Lu^b

a College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001;
b School of Material and Chemical Engineering, Henan University of Engineering, Zhengzhou 450007;
c College of Chemical Engineering and Food Science, Zhengzhou Institute of Technology, Zhengzhou 450044

Received:2017-08-21 Revised:2017-10-26 Published:2017-10-31
Contact: 10.6023/cjoc201708041 E-mail:luckyluke@haue.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21572046, 21172054).

1. Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51.PDF(972KB)

Abstract

Peptide breast cancer susceptibility gene BRCA1 plays an active role in inhibiting female breast cancer. The interaction between BRCA1 and breast cancer suppressor gene protein RAD51 in cancer cell is also an essential part for the treatment of breast cancer. Discovery Studio simulation of the docking process of BRCA1 analogs and RAD51 was used to screen the BRCA1 analogs with different charge and acid-base properties. The R-DOCK evaluation system is used to screened out 4 higher BRCA1 like peptides from the results, and the interactions with BRCA1 analogs of RAD51 peptides (Pep158-180, Pep181-200 and Pep241-260) were studied by using fluorescence spectroscopy and CD spectroscopy. The results indicate that acid-base properties of BRCA1 analogs have great influence on their interaction. The results showed that the effect of BRCA1-3 to RAD51 (Pep158-180/Pep241-260) which is the two key peptides were obviously higher than the other parent peptide. Besides, compared with BRCA1, all BRCA1 analogs combined with RAD51 much closer. The results provide evidence to design novel breast drugs for breast cancer.

Key words: BRCA1 peptide, RAD51 peptide, fluorescence, CD spectroscopy, DS simulation

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Li Linlu, Lü Mingxiu, Lu Kui, Liu Guangbin, Peng Lu. Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51[J]. Chin. J. Org. Chem., 2018, 38(1): 246-252.

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类乳腺癌易感基因BRCA1肽的设计、合成及与抑癌基因蛋白RAD51肽段的相互作用

Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51

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