Chinese Journal of Organic Chemistry ›› 2024, Vol. 44 ›› Issue (4): 1240-1246.DOI: 10.6023/cjoc202309008 Previous Articles     Next Articles

ARTICLES

脂滴特异性荧光探针的构建及可视化肝肿瘤细胞

徐冬青*(), 童海姗, 沈杰, 邱万伟, 钱立生   

  1. 安徽科技学院生命与健康科学学院 安徽凤阳 233100
  • 收稿日期:2023-09-08 修回日期:2023-11-29 发布日期:2023-12-21
  • 基金资助:
    安徽省教育厅重点项目(2023AH051883); 安徽省教育厅重点项目(2022AH051632); 大连金点生物科技有限公司资助的横向项目(880988); 上海勍臻检测技术有限公司(881242)

Construction of a Lipid Droplets Targeted Fluorescent Probe for Visualization of Liver Tumor Cells

Dongqing Xu(), Haishan Tong, Jie Shen, Wanwei Qiu, Lisheng Qian   

  1. School of Life and Health Science, Anhui Science and Technology University, Fengyang, Anhui 233100
  • Received:2023-09-08 Revised:2023-11-29 Published:2023-12-21
  • Contact: E-mail: xudongq@163.com
  • Supported by:
    Key Project of Anhui Provincial Department of Education(2023AH051883); Key Project of Anhui Provincial Department of Education(2022AH051632); Horizontal Project from Dalian Jindian Biotechnology Co., Ltd(880988); Shanghai Qingzhen Test Technology Co., Ltd(881242)

Lipid droplets (LDs) are involved in the metabolic processes of many living cells, and their anomalies are highly associated with a variety of diseases, such as fatty liver, diabetes and cancers. But it is still a great challenge to elucidate the intrinsic relationship between various diseases and LDs. In particular, there has been only a few studies on the visualization of abnormal LDs in liver tumor cells. Therefore, in this paper, a lipid droplets targeted fluorescent probe was easily prepared through a two-step procedure by using triphenylamine as the electron donor and polycyanide heterocyclic compound as the electron acceptor. The fluorescent probe TCN-Tph possesses good ability to target LDs in living cells through their co- localization imaging experiments. In comparison with other carcinoma cells, the liver tumor cells have good response and uptake of TCN-Tph. The slices images of liver tissues in different states further show that the probe TCN-Tph can also distinguish liver tissues and normal liver tissues effectively, which provide an experimental basis for the early diagnosis of liver tumor cells in clinic.

Key words: lipid droplets, fluorescent probe, liver tumor cell, visualization