Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (10): 2720-2730.DOI: 10.6023/cjoc201803048 Previous Articles     Next Articles

Special Issue: 荧光探针-生物传感合辑



张成路, 李益政, 李静怡, 李奕嶙, 宫荣庆, 王华玉   

  1. 辽宁师范大学化学化工学院 大连 116029
  • 收稿日期:2018-03-28 修回日期:2018-06-07 发布日期:2018-06-29
  • 通讯作者: 张成路,
  • 基金资助:


Synthesis and Bioactivities of Novel 1,2,4-Triazine Scheleton Phenanthroline Derivatives and the Fluorescent Recognition on DNA Using Three Novel Co (III) Complexes

Zhang Chenglu, Li Yizheng, Li Jingyi, Li Yilin, Gong Rongqing, Wang Huayu   

  1. School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029
  • Received:2018-03-28 Revised:2018-06-07 Published:2018-06-29
  • Contact: 10.6023/cjoc201803048
  • Supported by:

    Project supported by the Science and Technology Research Program of Liaoning Provincial Department of Education (No. 2009A426).

Cdc25B has become the important target for curing cancer owing to its over expression in kinds of cancers. Compounds containing phenanthroline moiety have become research objectives on the DNA fluorescence probes or the new curing agents for their excellent fluorescence property and bioactivities. Twelve novel 1,2,4-triazine scheleton phenanthroling derivatives ARTP1ARTP12 were first designed and synthesized, the structures of ARTP1ARTP12 were characterized successfully by means of IR and NMR. The inhibitory activities of ARTP1ARTP12 against Cdc25B were evaluated. The results show that nine target molecules exhibit excellent inhibitories, four molecules behave better activities than the contrast reference Na3VO4 indicating that they may be used as Cdc25B inhibitors. Meanwhile, three novel complexes Co-ARTP-5, Co-ARTP-6 and Co-ARTP-10 were first afforded by the reaction of the excellent inhibitory active compounds ARTP5, ARTP6 and ARTP10 with Co3+ respectively. The structures of the three complexes were confirmed through IR, UV-Vis, 1H NMR and fluorescence spectra. The interaction modes between the complexes and CT-DNA were explored. As a result, the excitation peaks of the complexes show a red shift and the complexes interact with CT-DNA through the insert mode. The binding constants Kb are (2.12±0.20)×105, (3.29±0.20)×105and (1.50±0.20)×105 L·mol-1, respectively, and it occurs strong fluorescene quenching. The complexes are expected to be the DNA fluorescence probes.

Key words: 1,10-phenanthroline, 1,2,4-triazine, Cdc25B, Co(III) complex, fluorescent