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化学学报
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化学学报 ›› 2001, Vol. 59 ›› Issue (10): 1614-1618. 上一篇    下一篇

研究论文

三七水煎液中还原型谷胱甘肽及腺嘌呤的分离与鉴定

纪建国;叶蕴华;邢其毅   

  1. 北京大学化学与分子工程学院生物有机与分子工程教育部;重点实验室
  • 发布日期:2001-10-15

Isolation and identification of reduced glutathione and adenine in the boiling water extract of panax notoginseng

Ji Jianguo;Ye Yunhua;Xing Qiyi   

  • Published:2001-10-15

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393

被引次数

1

三七经水煎提取，阴、阳离子交换树脂分离以及RP-HPLC分离获得多种含肽类化合物组分，采用游离及酸水解后氨基酸组成及含量对照分析的方法预示了提取液中肽类化合物的存在，经氨基酸组成分析、聚丙烯酰胺薄膜双向层析、羧肽酶C-末端序列分析、质谱测定等方法分析，将YN-3H12组分鉴定为还原型谷胱甘肽，YN-3H11组分鉴定为腺嘌呤，本研究方法可为植物中水溶性肽类化合物等活性成分的研究提供参考。

关键词: 三七, 谷胱甘肽, 腺嘌呤, 分离, 高速液体色谱

Many peptide constituents were separated from the boiling water extract of Panax notoginseng by extraction, anion and cation ion exchange resin separation and RP-HPLC separation methods. Peptide components were predicted to be existed in the boiling water extract by comparison of the composition and content of free amino acid and that after hydrolysis. YN-3H12 obtained from the boiling water extract of Panax notoginseng was identified as reduced glutathione by amino acid analysis, two dimentional chromatogram of polyacrylamide film, C-terminal analysis by carboxypeptidase digestion and mass chromatography analysis, The compound YN-3H11 was determined as adenine by analysis of its 1^H-, 13^C-NMR, MS spectra and by comparison with an authentic sample. This experiment provided an effective method to study the water-soluble peptide compounds and other bioactive components in the plants.

Key words: PANAX PSEUDOGINSENG VAR. NOTOGINSENG, GLUTATHIONE, ADENINE, SEPARATION, HIGH SPEED LIQUID CHROMATOGRAPHY

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