Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (4): 692-697. Previous Articles     Next Articles

Original Articles

应用于检测体液中葡萄糖含量的微生物传感器研究

朱龙;李元宗;慈云祥;M   

  1. 北京大学化学与分子工程学院.北京(100871);邓家祺,分析化学前沿(1991)
  • 发布日期:2002-04-15

Microbial Based Sensor for the Measurement of Glucose in Serum and Urine

Zhu Long;Li Yuanzong;Ci Yunxiang   

  1. College of Chemistry and Molecular Engineering, Peking University. Beijing(100871)
  • Published:2002-04-15

A microbial based biosensor for the measurement of glucose in serum and urine has been developed. Bscillus licheniformis, a microbial, was used in the cytosensor as the producer of glucose oxidase that are required in the biosensor. The influence of the types of microbes, enzyme immobilizing techniques and the carrier for the enzyme or microbial were studied. The new sensor has the advantages of high stability, low cost and easier preservation. It is the first time to develop a cytosensor with a mimic enzyme catalyzed fluorescence reaction for the measurement of glucose in serum and urine.

Key words: SENSORS, GLUCOSE OXIDASE, BACILLUS, REACTION MECHANISM, FLUORIMETRIC ANALYSIS

CLC Number: