有机化学 ›› 2018, Vol. 38 ›› Issue (9): 2281-2295.DOI: 10.6023/cjoc201806004 上一篇    下一篇

所属专题: 合成科学

综述与进展

鼠李糖基转移酶研究进展

闫雅如, 齐博文, 莫婷, 王晓晖, 王娟, 史社坡, 刘晓, 屠鹏飞   

  1. 北京中医药大学中药学院 中药现代研究中心 北京 100029
  • 收稿日期:2018-06-01 修回日期:2018-08-10 发布日期:2018-08-14
  • 通讯作者: 刘晓, 屠鹏飞, 史社坡 E-mail:fcliuxiao@163.com;pengfeitu@163.com;shishepo@163.com
  • 基金资助:

    国家自然科学基金(No.81402809)及北京中医药大学杰出青年人才(No.2018-JYB-XJQ006)资助项目.

Research Progress of Rhamnosyltransferase

Yan Yaru, Qi Bowen, Mo Ting, Wang Xiaohui, Wang Juan, Shi Shepo, Liu Xiao, Tu Pengfei   

  1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Meteria Medica, Beijing University of Chinese Medicine, Beijing 100029
  • Received:2018-06-01 Revised:2018-08-10 Published:2018-08-14
  • Contact: 10.6023/cjoc201806004 E-mail:fcliuxiao@163.com;pengfeitu@163.com;shishepo@163.com
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 81402809) and the Foundation from Beijing University of Chinese Medicine (No. 2018-JYB-XJQ006).

鼠李糖基化属于糖基化反应的一种,是化合物结构修饰及有机合成中广泛涉及到的一类反应.鼠李糖基转移酶是生物体中催化这一类反应的酶,能够将活性鼠李糖基从核苷糖转移到特定受体.这类酶广泛存在于自然界中,参与次生代谢产物的生成,并在生物体的结构组成及多种生理功能中发挥重要的作用.而且,得益于酶催化鼠李糖基化的特异性强、反应条件温和、环境友好等优点,其往往成为化学催化的有力补充,在有机化学合成及苷化修饰中发挥越来越重要的作用.对植物及微生物来源的鼠李糖基转移酶从酶催化功能、蛋白三维结构、鼠李糖基供体合成、酶催化底物杂泛性以及其在催化合成中的应用等方面进行了综述,并对其未来发展趋势进行了展望.

关键词: 鼠李糖基转移酶, 生物合成, 酶催化, 生物催化

Rhamnosylation is an important type of glycosylation reaction which is widely involved in organic synthesis and structural modification of natural products. In vivo, rhamnosylation is catalyzed by rhamnosyltransferase that transferred the active rhamnosyl donors to the specific sugar acceptors. A larger number of rhamnosyltransferases have been identified in natural and they often played key roles in the biosynthesis of diverse natural products as well as maintaining the cell structure and physiological functions of biological organisms. Besides, enzymatic rhamnosylation has been an effective complementary method to chemical catalysis in the field of organic glycosylation modifications due to its high catalysis efficiency and specificity, mild reaction conditions as well as environment friendship and so on. In this article, research progresses of rhamnosyltransferase are reviewed based on their enzymatic functions, three dimensional structure investigations, rhamnosyl donors' synthesis, enzymatic catalysis promiscuities, and biochemical catalysis applications. Finally, the future development and application of them are also prospected.

Key words: rhamnosyltransferase, biosynthesis, enzyme catalysis, biocatalysis