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研究论文

一种比色/荧光快速检测SO₂衍生物的近红外荧光探针及其应用与成像

尹婕a, 田利a, 梁天宇a, 钟克利a,*, 燕小梅b, 汤立军a,*   

  1. a渤海大学化学学院,辽宁省高等学校应用化学重点实验室,锦州 121013;
    b大连医科大学检验医学院 大连 116044
  • 收稿日期:2026-04-18 修回日期:2026-06-03
  • 基金资助:
    国家自然科学基金（No. 22278038）、渤海大学海洋研究所开放项目（No. BDHYYJY2025003, BDHYYJY2025008）、辽宁省教育厅科研基金项目（Nos. JTTMS1618）资助.

A near-infrared fluorescent probe for rapid colorimetric/fluorescent detection of SO₂ derivatives and its application and imaging

Yin Jiea, Tian Lia, Liang Tianyua, Zhong Kelia,*, Yan Xiaomeib, Tang Lijuna,*   

  1. aCollege of Chemistry, Liaoning Provincial Key Laboratory of Applied Chemistry for Institutions of Higher Education, Bohai University, Jinzhou 121013;
    bCollege of Medical Laboratory, Dalian Medical University, Dalian, 116044
  • Received:2026-04-18 Revised:2026-06-03
  • Contact: * E-mail: zhongkeli2000@bhu.edu.cn ; ljtang@bhu.edu.cn.
  • Supported by:
    National Natural Science Foundation of China (No. 22278038); the Open Fund of Institute of Ocean Research of Bohai University (No. BDHYYJY2025003, BDHYYJY2025008); Scientific Research Project of Liaoning Provincial Department of Education (Nos. JTTMS1618).

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补充材料

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本文将6-(二乙氨基)-1,2-二氢环戊并[b]色烯醛与1,3-茚满二酮进行亲核加成,合成了一种新颖的具有近红外发射(720 nm)的荧光探针Did。该探针能在DMF/PBS (5/5, v/v)溶液中快速(40 s)识别SO₂衍生物,具有选择性优异,抗干扰能力强,检测限低 (5.29 μmol/L)等优点,同时肉眼可观察到溶液颜色由蓝绿色迅速褪至无色,可裸眼识别。此外,探针Did可检测白糖、红酒和饼干等真实食物样品中的HSO3-,并可对活细胞中HSO3-进行荧光成像,在食品安全和生物成像领域具有潜在的应用潜力。

关键词: 近红外发射, 荧光探针, 亚硫酸氢盐, 生物成像, 食品样品

A novel near-infrared (NIR) fluorescent probe Did with an emission wavelength of 720 nm, was synthesized via the nucleophilic addition reaction between 6-(diethylamino)-1,2-dihydrocyclopenta[b] chromen-1-carbaldehyde and 1,3-indanedione. In DMF/PBS (5/5, v/v) solution, the probe exhibited rapid recognition (40 s) towards sulfur dioxide derivatives, featuring excellent selectivity, strong anti-interference ability, and a low detection limit of 5.29 μmol/L. Meanwhile, a distinct color change from cyan to colorless could be visually observed by the naked eye, enabling naked-eye identification. Furthermore, probe Did was successfully applied to detect HSO₃⁻ in real food samples such as white sugar, red wine, biscuits, and also realized fluorescent imaging of HSO₃⁻ in living cells. These results demonstrate that probe Did has great potential application prospects in the fields of food safety, and biological imaging.

Key words: NIR emission, Fluorescence probe, Bisulfite, Bioimaging, Food sample