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有机化学
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有机化学 ›› 2009, Vol. 29 ›› Issue (04): 580-583. 上一篇    下一篇

研究论文

多羟乙基双核大环多胺La(III)配合物对双对硝基苯酚磷酸二酯和DNA 催化水解作用研究

向清祥*; 张丽群; 余孝其; 谢如刚   

  1. (a乐山师范学院化学与生命科学学院 乐山 614004)
    (b四川大学化学学院 成都 610064)
  • 收稿日期:2008-09-19 修回日期:2008-10-20 发布日期:2009-04-20
  • 通讯作者: 向清祥

Catalytic Hydrolysis of Bis(p-nitrophenyl)phosphate Diester and Plasmid DNA by Dinuclear Macrocyclic Polyamine La(III) Complexes Bearing Multiethoxyl Groups

Xiang, Qingxiang*; Zhang, Liqun; Yu, Xiaoqi ; Xie, Rugang

  

  1. (a Department of Chemistry and Life Science, Leshan Teachers College, Leshan 614004)
    (b Department of Chemistry, Sichuan University, Chengdu 610064)
  • Received:2008-09-19 Revised:2008-10-20 Published:2009-04-20
  • Contact: Xiang, Qingxiang

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1032

被引次数

3

合成了一类新型多羟乙基双核大环多胺La(III)配合物, 其结构经1H NMR, MS, 元素分析等表征. 通过紫外分光光谱法和琼脂糖凝胶电泳技术, 研究了双核大环多胺La(III)配合物催化双对硝基苯酚磷酸二酯的水解反应和对质粒DNA(pUC18)的催化水解作用. 结果表明: 双核大环多胺La(III)配合物可以有效催化双对硝基苯酚磷酸二酯水解和促进质粒DNA在生理条件下的水解裂解. 双对硝基苯酚磷酸二酯的水解速率提高了2.36×104倍. 讨论了配合物结构对水解反应的影响.

关键词: 双核大环多胺, 配合物, DNA, 双对硝基苯酚磷酸二酯水解

A series of novel dinuclear macrocyclic polyamine La(III) complexes bearing multiethoxyl groups have been synthesized in this paper and their structures were characterized by 1H NMR, MS techniques and elemental analysis. The hydrolysis of bis(p-nitrophenyl)phosphate diester and the cleavage of plasmid DNA (pUC18) have been investigated by UV-visible spectrophotometric titration and the agarose gel electrophoresis. The results indicated that these La(III) complexes could effectively catalyze the hydrolysis of bis(p-nitrophenyl)phosphate diester and the cleavage of plasmid DNA (pUC18) under physiological conditions. The hydrolysis rate enhancement of bis(p-nitrophenyl)phosphate diester was up to 2.36×104 fold. The effects of the structure on the hydrolytic reactions were discussed.

Key words: dinuclear macrocyclic polyamine, complex, hydrolysis of bis(p-nitrophenyl)phosphate diester, DNA