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有机化学
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有机化学 ›› 2001, Vol. 21 ›› Issue (7): 518-523. 上一篇    下一篇

研究论文

绿茶多酚对自由基诱导的红细胞膜过氧化的抑制作用

马兰萍;蔡育军;杨立;刘中立   

  1. 兰州大学应用有机化学国家重点实验室.兰州(730000)
  • 发布日期:2001-07-25

Protective effects of green tea polyphenols against free radical initiated peroxidation on erythrocyte ghosts

Ma Lanping;Cai Yujun;Yang Li;Liu Zhongli   

  1. Lanzhou Univ, State Key Lab Appl Organ Chem.Lanzhou(730000)
  • Published:2001-07-25

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386

被引次数

11 | 4

采用水溶性偶氮引发剂2,2'-偶氮二(2-脒基丙烷)二盐酸盐(AAPH)在37引发入血红细胞膜的过氧化，通过测定氧气吸收及维生素E的消耗研究了过氧化过程的动力学，并对从绿茶中提取的主要多酚类化合物的抗氧化活性做了定量研究。使用的绿茶多酚有:(-)-表儿茶素(EC),(-)-表儿茶素(EGC),(-)-表儿茶素酸酯(ECG)和(-)-表儿茶素培酸酯(EGCG)。结果表明，这些绿茶多酚能够显著缩短过氧化反应的动力学链长，有效地抑制红细胞膜的过氧化。抗氧化活性顺序为:EC〉GCG〉EGCG〉EGC。

关键词: 绿茶多酚, 抗氧化剂, 自由基, 红细胞膜, 脒, 诱导, 抗氧化剂

Peroxidation of human erythrocyte ghosts was initiated by a water- soluble free radical initiator 2,2'-azo(2-amidinopropane) dihydrochloride (AAPH). The peroxidation was monitored by oxygen uptake and consumpotion of vitamin E. It was found that the peroxidation was inhibited by the principal polyphenolic components extracted from green tea leaves, i. e. (-)-epicatechin (EC), (-)- epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)- epigallocatechin gallate (EGCG). Kinetic analysis of the antioxidation process demonstrated that these green tea polyphenols could significantly shorten the kinetic chain length of the peroxidation and inhibit the peroxidation of erythrocyte ghosts with the activity sequence of EC〉GCG〉EGCG〉EGC.

Key words: ANTIOXIDANTS, FREE RADICALS, AMIDINE, INDUCTION, ANTIOXIDANTS

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