Chin. J. Org. Chem. ›› 2016, Vol. 36 ›› Issue (6): 1401-1406.DOI: 10.6023/cjoc201512033 Previous Articles     Next Articles



张应鹏a, 尤彩霞a, 杨云裳a, 刘小育a, 郭慧琛b, 董玉莹a   

  1. a 兰州理工大学石油化工学院兰州 730050;
    b 中国农业科学院兰州兽医研究所兰州 730050
  • 收稿日期:2015-12-23 修回日期:2016-01-15 发布日期:2016-02-24
  • 通讯作者: 张应鹏, 杨云裳;
  • 基金资助:


Synthesis of Reversible Fluorescence Quenched Probe for Cu2+ and Imaging in PK-15 cells

Zhang Yingpenga, You Caixiaa, Yang Yunshanga, Liu Xiaoyua, Guo Huichenb, Dong Yuyinga   

  1. aSchool of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050;
    b Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730050
  • Received:2015-12-23 Revised:2016-01-15 Published:2016-02-24
  • Supported by:

    Project supported by the Natural Science Foundation of Gansu Province (No. 2014GS03265 ).

A new pyrazoline derivative 1-(2-hydroxyphenyl)-4-(9-anthryl)-3-benzothiazole pyrazolin (P1) was designed and synthesized. The recognition properties of the target compound P1 with metal ions had been investigated by UV absorption and fluorescence spectrophotometry. The probe exhibits several favorable features, including instantaneous, reversible recognition, high sensitivity and selectivity to copper ions. Cytotoxicity test and fluorescent immunoassay results show that compound P1 has good biocompatibility and low toxicity with its IC50 value of 376.558 μmol/L.

Key words: pyrazoline, fluorescent probe, copper ions, recognition, PK-15 living cells