Synthesis of a Reversible Fluorescence Probe for Hg2+ and Its Application in Living Cells

doi:10.6023/cjoc201403009

Chin. J. Org. Chem. ›› 2014, Vol. 34 ›› Issue (8): 1646-1651.DOI: 10.6023/cjoc201403009 Previous Articles Next Articles

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可逆识别Hg²⁺荧光探针的合成及活细胞中成像的应用

周佳, 杨美盼, 孟文斐, 成昭, 杨秉勤

合成与天然功能分子化学教育部重点实验室西北大学化学与材料科学学院西安 710069

收稿日期:2014-03-05 修回日期:2014-03-28 发布日期:2014-04-15
通讯作者: 杨秉勤 E-mail:yangbq@nwu.edu.cn
基金资助:
国家自然科学基金（No.21172178）资助项目.

Synthesis of a Reversible Fluorescence Probe for Hg²⁺ and Its Application in Living Cells

Zhou Jia, Yang Meipan, Meng Wenfei, Cheng Zhao, Yang Bingqin

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710069

Received:2014-03-05 Revised:2014-03-28 Published:2014-04-15
Supported by:
Project supported by the National Natural Science Foundation of China (No. 21172178).

A new rhodamine B derivative T1 has been rationally designed and synthesized facilely by the condensation reaction of rhodamine B hydrazide and o-phthaloyl chloride. The recognition properties of the target compound T1 with metal ions had been investigated by the fluorescence spectrophotometry. This probe exhibits several favorable features, including instantaneous and reversible recognition, high sensitivity and selectivity to mercury ions. When the concentration of Hg²⁺ was in the range of 4×10^-8～9×10^-8 mol/L, there was a good linearity between the fluorescence intensity and the concentration of Hg²⁺. The fluorescence imaging experiments of Hg²⁺ in HepG2 living cells demonstrated their valuable application in biological system.

Key words: rhodamine B, fluorescent probe, mercury ions, recognition, HepG2 living cells

Cite this article

Zhou Jia, Yang Meipan, Meng Wenfei, Cheng Zhao, Yang Bingqin. Synthesis of a Reversible Fluorescence Probe for Hg²⁺ and Its Application in Living Cells[J]. Chin. J. Org. Chem., 2014, 34(8): 1646-1651.

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可逆识别Hg²⁺荧光探针的合成及活细胞中成像的应用

Synthesis of a Reversible Fluorescence Probe for Hg²⁺ and Its Application in Living Cells

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可逆识别Hg2+荧光探针的合成及活细胞中成像的应用