Synthesis of Reversible Fluorescence Quenched Probe for Cu2+ and Imaging in PK-15 cells

doi:10.6023/cjoc201512033

Chin. J. Org. Chem. ›› 2016, Vol. 36 ›› Issue (6): 1401-1406.DOI: 10.6023/cjoc201512033 Previous Articles Next Articles

Notes

可逆识别Cu²⁺的吡唑啉荧光猝灭型探针及其在PK-15细胞成像中的应用

张应鹏^a, 尤彩霞^a, 杨云裳^a, 刘小育^a, 郭慧琛^b, 董玉莹^a

a 兰州理工大学石油化工学院兰州 730050;
b 中国农业科学院兰州兽医研究所兰州 730050

收稿日期:2015-12-23 修回日期:2016-01-15 发布日期:2016-02-24
通讯作者: 张应鹏, 杨云裳 E-mail:yingpengzhang@126.com;yangyunshang@tom.com
基金资助:
甘肃省自然科学基金(No.2014GS03265)资助项目.

Synthesis of Reversible Fluorescence Quenched Probe for Cu²⁺ and Imaging in PK-15 cells

Zhang Yingpeng^a, You Caixia^a, Yang Yunshang^a, Liu Xiaoyu^a, Guo Huichen^b, Dong Yuying^a

aSchool of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050;
b Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730050

Received:2015-12-23 Revised:2016-01-15 Published:2016-02-24
Supported by:
Project supported by the Natural Science Foundation of Gansu Province (No. 2014GS03265 ).

1. Synthesis of Reversible Fluorescence Quenched Probe for Cu²⁺ and Imaging in PK-15 cells.PDF(281KB)

Abstract

A new pyrazoline derivative 1-(2-hydroxyphenyl)-4-(9-anthryl)-3-benzothiazole pyrazolin (P1) was designed and synthesized. The recognition properties of the target compound P1 with metal ions had been investigated by UV absorption and fluorescence spectrophotometry. The probe exhibits several favorable features, including instantaneous, reversible recognition, high sensitivity and selectivity to copper ions. Cytotoxicity test and fluorescent immunoassay results show that compound P1 has good biocompatibility and low toxicity with its IC₅₀value of 376.558 μmol/L.

Key words: pyrazoline, fluorescent probe, copper ions, recognition, PK-15 living cells

Cite this article

Zhang Yingpeng, You Caixia, Yang Yunshang, Liu Xiaoyu, Guo Huichen, Dong Yuying. Synthesis of Reversible Fluorescence Quenched Probe for Cu²⁺ and Imaging in PK-15 cells[J]. Chin. J. Org. Chem., 2016, 36(6): 1401-1406.

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可逆识别Cu²⁺的吡唑啉荧光猝灭型探针及其在PK-15细胞成像中的应用

Synthesis of Reversible Fluorescence Quenched Probe for Cu²⁺ and Imaging in PK-15 cells

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可逆识别Cu2+的吡唑啉荧光猝灭型探针及其在PK-15细胞成像中的应用