Chinese Journal of Organic Chemistry ›› 2022, Vol. 42 ›› Issue (9): 2850-2856.DOI: 10.6023/cjoc202203039 Previous Articles     Next Articles

ARTICLES

溶酶体靶向远红光至近红外荧光探针用于监测铁死亡过程中粘度的变化

赖燕琴a, 陈雪a, 陈芳a, 倪林晨a, 王婷b, 祝子坪a, 满菊a, 姜春筱a, 谢振达a,*()   

  1. a 台州学院生命科学学院 浙江台州 318000
    b 台州学院医学院 浙江台州 318000
  • 收稿日期:2022-03-21 修回日期:2022-05-29 发布日期:2022-06-16
  • 通讯作者: 谢振达
  • 基金资助:
    台州市科技项目(2003gy14); 台州市科技项目(21ywb80); 台州学院科研项目(2019PY021); 国家大学生创新创业训练计划(202010350033)

A Lysosome-Targeted Far-Red to Near-Infrared Fluorescent Probe for Monitoring Viscosity Change During the Ferroptosis Process

Yanqin Laia, Xue Chena, Fang Chena, Linchen Nia, Ting Wangb, Ziping Zhua, Ju Mana, Chunxiao Jianga, Zhenda Xiea()   

  1. a School of Life Science, Taizhou University, Taizhou, Zhejiang 318000
    b Medical College, Taizhou University, Taizhou, Zhejiang 318000
  • Received:2022-03-21 Revised:2022-05-29 Published:2022-06-16
  • Contact: Zhenda Xie
  • Supported by:
    Taizhou Science and Technology Project(2003gy14); Taizhou Science and Technology Project(21ywb80); Scientific Research Project of Taizhou University(2019PY021); National College Students Innovation and Entrepreneurship Training Program(202010350033)

Elucidating the intrinsic complex relationship between pathophysiological processes and lysosomal viscosity is a tremendous challenge. So far, the change of lysosomal viscosity during the ferroptosis process has not been reported. In this study, a novel far-red to near-infrared viscosity fluorescent probe (FNVP) was designed and synthesized based on boron dipyrromethene (BODIPY) dye. The probe exhibited excellent responsiveness to viscosity change (λex=600 nm, λem=680 nm). In the phosphate buffered saline (PBS)-glycerol system (1~219 cP), the emission intensity of probe FNVP at 680 nm had about a 50-times increase. Furthermore, the probe showed the advantages of high selectivity, remarkable photostability and negligible cytotoxicity. Probe FNVP was lysosome-targetable and could be used for monitoring lysosomal viscosity changes induced by nystatin. Finally, using confocal fluorescence microscopy, lysosome viscosity was imaged during the ferroptosis process at the cellular level. All those will be beneficial to further comprehending the biological effectiveness of ferroptosis.

Key words: viscosity, fluorescent probe, lysosome, near-infrared, ferroptosis