Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (9): 2467-2484.DOI: 10.6023/cjoc201901024 Previous Articles     Next Articles

REVIEW

生物小分子成像用双光子荧光探针

黄池宝a,b, 陈会a, 李福琴a, 安思雅a   

  1. a 遵义师范学院化学化工学院 遵义 563002;
    b 韶关大学农业科学与工程学院 韶关 512005
  • 收稿日期:2019-01-17 修回日期:2019-02-24 出版日期:2019-09-25 发布日期:2019-04-08
  • 通讯作者: 黄池宝 E-mail:huangchibao@163.com
  • 基金资助:

    贵州省高层次创新型人才培养计划项目——"百"层次人才(No.[2016]5683)、国家自然科学基金(No.21562050)、贵州省第八批科技创新人才团队建设专项基金[No.(2015)4007]、贵州省科学技术基金(No.J[2015]2146)、贵州省教育厅自然科学重点研究(No.KY[2014]296)和贵州省教育厅省级本科教学工程建设(No.KY[2014]JXGChcb)资助项目.

Two-Photon Fluorescence Probes for Small Biomolecules Imaging

Huang Chibaoa,b, Chen Huia, Li Fuqina, An Siyaa   

  1. a Chemistry and Chemical Engineering College, Zunyi Normal University, Zunyi 563002;
    b College of Agricultural Science and Engineering, Shaoguan University, Shaoguan 512005
  • Received:2019-01-17 Revised:2019-02-24 Online:2019-09-25 Published:2019-04-08
  • Contact: 10.6023/cjoc201901024 E-mail:huangchibao@163.com
  • Supported by:

    Project supported by the Guizhou Province High-level Innovative Talents Training Project-Hundred Talents Program (No.[2016]5683), the National Natural Science Foundation of China (No. 21562050), the Special Fund Project of the Construction of the Eighth Batch of Scientific and Technological Innovation Talent Team in Guizhou Province[No. (2015)4007], the Guizhou Science and Technology Fund Project (No. J[2015]2146), the Key Project of Education Department of Guizhou Province (No. KY[2014]296) and the Teaching Contents and Curriculum System Reform Project of Higher Education in Guizhou Province (No. KY[2014] JXGChcb).

Small molecules (biological small molecules) in vivo are not only in a large number, involving inorganic small molecules, such as SO2, H2S, NO and CO, and more organic small molecules, such as monosaccharides, oligosaccharides, hormones, coenzymes, glycerol, stimulating factors, regulatory factors, Vitamins, etc., moreover, play an important role in pathology, physiology, and so on. Therefore, it is necessary to observe and monitor small molecules in vivo in real time, and the two-photon fluorescence probe is a necessary tool to achieve this goal. The advantages of two-photon fluorescence probe, such as fixed target (very small dot) excitation, high horizontal and vertical resolution, no photobleaching, no phototoxicity and deep imaging in tissues, and so on, demonstrate its unparalleled superiority. It can be used for dynamic 3D observation and monitoring of biological small molecules in cells or tissues. In this paper, CO, monosaccharide (glucose, β-galactosidase), SO2, H2S, NO, peroxy (sulfur) compounds, mercaptan/thiophenol, 1O2, formaldehyde, HNO, HclO, O2·- and ONOO- two-photon fluorescence probes which have been developed in recent 5 years were reviewed. The sensing mechanisms of these two-photon fluorescence probes were systematically analyzed, and the development and prospect of two-photon fluorescence probes for small biomolecules are prospected.

Key words: biological small molecule, fluorescent probe, sensing mechanism, fluorophore, receptor, two-photon bioimaging