Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (8): 2502-2507.DOI: 10.6023/cjoc202003007 Previous Articles     Next Articles

一种新型比率型荧光探针用于细胞内半胱氨酸的检测

周小琴a, 崔梦园b, 贾程利a, 杨敏a, 吉民a, 王鹏b   

  1. a 东南大学生物科学与医学工程学院 南京 210009;
    b 中国药科大学生物医学工程学院 南京 211198
  • 收稿日期:2020-03-03 修回日期:2020-05-04 出版日期:2020-08-25 发布日期:2020-05-20
  • 通讯作者: 吉民, 王鹏 E-mail:101010516@seu.edu.cn;wangpeng159seu@hotmail.com
  • 基金资助:
    国家自然科学基金(No.81671745)资助项目.

Novel Ratio-Based Fluorescent Probe for Intracellular Cys Detection

Zhou Xiaoqina, Cui Mengyuanb, Jia Chenglia, Yang Mina, Ji Mina, Wang Pengb   

  1. a School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210009;
    b School of Biomedical Engineering, China Pharmaceutical University, Nanjing 211198
  • Received:2020-03-03 Revised:2020-05-04 Online:2020-08-25 Published:2020-05-20
  • Supported by:
    Project supported by the National Natural Science Foundation of China (No. 81671745).

Cysteine is an important biological component of the human body, its levels are related to many diseases. It is especially important to respond quickly and accurately to cysteine concentrations. This study is based on the classical cysteine response mechanism. The thiol group of cysteine undergoes Michael addition to acrylate, and then undergoes intramolecular cyclization to specifically recognize cysteine. A novel ratio-based fluorescent probe was designed and synthesized in this study. The research results found that the probe can specifically recognize cysteine, the detection limit can reach 75 nmol·L-1, can fully respond within 30 min, and has good stability. It exists in cells and has little cytotoxic effects. Therefore, on the basis of solving the problem of poor water solubility, the probe can quickly and accurately analyze the concentration of cysteine in cells, which provides a new means for the study of various diseases and new ideas for the development of chemical materials.

Key words: cysteine, ratio-based, fluorescent probe, michael addition, indocyanines