用于神经递质检测与生物成像的有机小分子荧光探针★

doi:10.6023/A25050200

化学学报 ›› 2025, Vol. 83 ›› Issue (10): 1252-1266.DOI: 10.6023/A25050200 上一篇下一篇

综述

用于神经递质检测与生物成像的有机小分子荧光探针^★

孟倩^a, 张琪伟^a^,^b^,^*()

^a 华东师范大学化学与分子工程学院上海市绿色化学与化工过程绿色化重点实验室上海 200241
^b 华东师范大学医学磁共振与分子影像技术研究院上海 200241

投稿日期:2025-05-31 发布日期:2025-08-12
通讯作者: 张琪伟

作者简介:

孟倩, 2024年毕业于阜阳师范大学, 获硕士学位. 现为华东师范大学博士研究生, 导师为张琪伟教授. 主要从事有机发光材料的设计合成及其分析与成像应用研究.

张琪伟, 华东师范大学化学与分子工程学院教授、博士生导师, 国家自然科学基金优秀青年科学基金获得者. 2005~2014年在华东理工大学获得学士与博士学位, 师从田禾院士. 2014~2018年, 先后在荷兰奈梅亨大学(Radboud University, 合作导师Roeland J.M. Nolte院士)和美国圣母大学(University of Notre Dame, 合作导师Bradley D. Smith教授)从事博士后研究. 2018年回国入职华东师范大学, 主要从事有机光功能材料、荧光传感与生物成像和光学诊疗等领域的研究.

^★ “中国青年化学家”专辑.

基金资助:
国家自然科学基金(22322405); 国家自然科学基金(22274055); 上海市基础研究特区计划(TQ20240206)

Organic Small-Molecule Fluorescent Probes for Neurotransmitter Sensing and Bioimaging^★

Qian Meng^a, Qiwei Zhang^a^,^b^,^*()

^a Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241
^b Institute of Magnetic Resonance and Molecular Imaging in Medicine, East China Normal University, Shanghai 200241

Received:2025-05-31 Published:2025-08-12
Contact: Qiwei Zhang
About author:
^★ For the VSI “Rising Stars in Chemistry”.
Supported by:
National Natural Science Foundation of China(22322405); National Natural Science Foundation of China(22274055); Shanghai Pilot Program for Basic Research(TQ20240206)

文章分享

摘要/Abstract

神经递质作为介导神经元间信息传递、调控神经环路及维持脑功能稳态的关键信使分子, 其动态监测对解析神经生理/病理机制具有重要意义. 有机小分子荧光探针以其优异的结构稳定性、生物相容性、识别机制的可设计性, 以及发光特性的灵活可调性, 为神经递质的检测和成像提供了理想的方式. 此文从有机小分子荧光探针的分子设计策略、共价/非共价识别原理、信号转导机制, 以及生物成像性能等方面出发, 对神经递质荧光探针的最新研究进展进行综述. 在此基础上, 对当前探针的局限性进行了深入分析, 并对未来新型探针的设计方向进行了展望, 旨在为新一代高性能神经化学探针的研发提供理论框架与路线图, 以促进神经科学和临床诊断技术的发展.

关键词: 荧光探针, 神经递质, 有机小分子, 超分子, 生物成像

Neurotransmitters are essential mediators of neuronal communication, governing neural circuit plasticity and maintaining cerebral functional homeostasis. They can be classified into major categories including monoamines, amino acids, acetylcholine, neuropeptides, purines, gaseous neurotransmitters, etc. Deciphering the dynamic fluctuations in neurotransmitter concentrations and distributions is paramount for elucidating fundamental neurophysiological processes and unraveling the mechanisms underpinning neuropathological conditions, such as Alzheimer's disease, Parkinson's disease, depression, etc. Fluorescence imaging technique employs specific probes that transduce the biochemical event of neurotransmitter recognition into a quantifiable optical signal, enabling non-invasive, highly specific visualization with high spatiotemporal resolution in live systems, representing an exceptionally promising class of tools for neurotransmitter detection. Owing to the compact size, excellent structural stability, high biocompatibility, tunable photophysical properties, and designable recognition mechanisms, organic small-molecule fluorescent probes act as ideal candidates for sensitive and selective neurochemical imaging. This review examines the advancements in the development and application of organic fluorescent probes for neurotransmitter detection, focusing on molecular designs, recognition principles (covalent or non-covalent interactions), signal transduction mechanisms (e.g., photoinduced electron transfer, intramolecular charge transfer, fluorescence resonance energy transfer), analytical performance, and their imaging capabilities in live cells, tissues, and in vivo models. A dedicated section provides a critical analysis of the prevailing limitations hindering broader application of current organic small-molecule fluorescent probes, such as challenges with reversibility, slow response times, photobleaching, limited multiplexing capability, insufficient blood-brain barrier penetration, and depth limitations in in vivo imaging. Building upon this assessment, we offer forward-looking perspectives on the future trajectory of fluorescent probe development. Key focus areas include engineering probes with enhanced binding kinetics and reversibility for real-time monitoring, superior photostability for longitudinal studies, capabilities for multiplexed analysis, ideal excitation/emission properties for deeper tissue penetration, and improved pharmacokinetic properties for efficient BBB crossing. This review aims to provide strategic design insights and a developmental roadmap for the next generation of high-performance neurochemical probes, thereby advancing fundamental neuroscience research and clinical diagnostic technologies.

Key words: fluorescent probes, neurotransmitters, organic small molecules, supermolecules, bioimaging

引用此文

孟倩, 张琪伟. 用于神经递质检测与生物成像的有机小分子荧光探针^★[J]. 化学学报, 2025, 83(10): 1252-1266.

Qian Meng, Qiwei Zhang. Organic Small-Molecule Fluorescent Probes for Neurotransmitter Sensing and Bioimaging^★[J]. Acta Chimica Sinica, 2025, 83(10): 1252-1266.

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

分享此文

图/表 13

图1. 综述中包含的小分子神经递质结构

Figure 1. The neurotransmitter structures involved in the review

图2. (a)探针1~3的化学结构和探针1对多巴胺的检测机制示意图; 探针4 (b), 5 (c)和PBA-CA (d)用于多巴胺识别的机制示意图

Figure 2. (a) Chemical structure of probes 1~3 and schematic diagram of the detection mechanism of probe 1 toward DA; schematic diagram of the mechanism of probes 4 (b), (c) 5 and (d) PBA-CA for the recognition of DA

图3. (a)探针6对去甲肾上腺素的检测机制示意图及传感器6~8的化学结构; (b)用探针6和PNMT抗体染色富含去甲肾上腺素和肾上腺素的混合嗜铬细胞图(绿色圆圈内的细胞表示NE富集细胞, 红色圆圈内的细胞表示EP富集细胞)[61]

Figure 3. (a) Schematic diagram of the detection mechanism of probe 6 toward NE, and chemical structures of probes 6~8; (b) stain mixed chromaffin cells rich in norepinephrine and adrenaline with probe 6 and PNMT antibody (cells circled in green indicate NE-enriched cells, and cells circled in red indicate EP-enriched cells)[61], Copyright 2013, American Chemical Society

图4. (a)探针检测去甲肾上腺素的级联亲核取代机制; (b)探针9~12的化学结构; (c)探针检测去甲肾上腺素的“狩猎-射击”策略; (d)小鼠脑荧光变化: (ⅰ)经尾静脉注射探针12后监测, (ⅱ)经尾血管注射探针12前(左)和后(右)监测, (ⅲ)通过解剖实验观察癫痫发作和非癫痫发作小鼠脑的荧光强度[68]

Figure 4. (a) Cascade nucleophilic substitution mechanism for probe detection of norepinephrine; (b) chemical structures of probes 9~12; (c) the "hunting-shooting" strategy for probe detection of norepinephrine; (d) changes in mice brain fluorescence (ⅰ) monitored after the injection of probe 12 via the tail vein, (ⅱ) before (left) and after (right) epileptic injection of probe 12 via tail vein, (ⅲ) fluorescence intensity of the mice brains with and without epileptic seizure by anatomical experiment[68], Copyright 2023, American Chemical Society

图5. (a)探针BPS3通过构象折叠和水桥的双重加速增强对去甲肾上腺素的超快传感机制示意图[69]; (b)探针BPS3对去甲肾上腺素的检测机制示意图[69]; (c)用共价或超分子方法进行分子构象调节的示意图. 探针BPS3的化学结构[69]; (d) BPS2、BPS3、BPS4和BPS3＋CB[7]水溶液的归一化荧光响应动力学, 插图: BPS3和BPS4对NE的响应的部分曲线的放大图[69]

Figure 5. (a) Schematic diagram of the ultrafast sensing mechanism of probe BPS3 for norepinephrine through dual acceleration of conformational folding and water bridging[69]; (b) schematic diagram of the detection mechanism of probe BPS3 toward NE[69]; (c) schematic of molecular conformational regulations by covalent or supramolecular approaches[69]; (d) normalized fluorescence response dynamics of aqueous solution of BPS2, BPS3, BPS4, and BPS3＋CB[7], Inset: enlarged view of the partial curve for the response of BPS3 and BPS4 toward NE[69], reproduced under a CC BY license

图6. (a)探针13的化学结构及其传感示意图[70]; (b)探针13a在含水量不同的乙腈-水混合溶剂中的反应动力学[70]; (c)通过计算得出在探针13a~13d和NE之间的亲核取代过程中, 可能反应坐标的相对能量[70]; (d)探针13a在正常小鼠、帕金森病(PD)模型小鼠以及经N-乙酰半胱氨酸(NAC)处理的PD模型小鼠的四个大脑区域组织切片荧光图像、体内成像以及相应的荧光变化柱状图[70]

Figure 6. (a) Chemical structures of probe 13 and its sensing schematic diagram[70]; (b) Reaction kinetics of probe 13a in acetonitrile-water mixed solvents with different water contents[70]; (c) Calculate the relative energy of possible reaction coordinates during the nucleophilic substitution process between probes 13a~13d and NE[70]; (d) Fluorescence images of tissue sections from four brain regions, in vivo imaging, and corresponding fluorescence change histograms of probe 13a in normal mice, Parkinson's disease (PD) model mice, and PD model mice treated with N-acetylcysteine (NAC) [70]. Copyright 2023, American Chemical Society

图7. (a)探针14的化学结构及优化的超分子传感器; (b)电刺激后用探针14孵育小鼠S1BF, CA1, LD和CPU区域的脑组织切片的荧光图像[88]

Figure 7. (a) Chemical structure of probe 14 and optimized supramolecular probe; (b) fluorescence images of brain tissue slices from S1BF, CA1, LD, and CPU regions incubated with probe 14 after electrical stimulation[88], Copyright 2025, American Chemical Society

图8. (a)探针15的化学结构及其对肾上腺素的检测机制示意图; (b)探针16的化学结构及其对肾上腺素的检测机制示意图[91]; (c) 26个不同脑区荧光信号的量化图[91]

Figure 8. (a) Chemical structure of probe 15 and schematic diagram of the detection mechanism toward EP; (b) chemical structure of probe 16 and its detection mechanism for EP[91]; (c) quantized maps of fluorescence signals in 26 different brain regions[91], reproduced under a CC BY license

图9. (a)探针17的化学结构示意图及其对5-HT的检测机制示意图; (b)探针18对5-HT的检测机制示意图

Figure 9. (a) Schematic diagram of the chemical structure of probe 17 and its detection mechanism for 5-HT; (b) Schematic diagram of the detection mechanism of probe 18 for 5-HT

图10. 探针19 (a)和20 (b)的化学结构及其对组胺的检测机制示意图

Figure 10. Chemical structures of probes 19 (a) and 20 (b), and schematic diagram of the detection mechanism toward HA

图11. (a)半隐烷1 (HC 1)的化学结构; (b)探针21的化学结构

Figure 11. (a) Chemical structure of hemicryptophane 1 (HC 1); (b) chemical structure of probe 21

图12. (a)探针22对谷氨酸和天冬氨酸的检测机制示意图及其在HeLa细胞中对天冬氨酸/谷氨酸的成像[113]; (b) 23的化学结构及其对谷氨酸和天冬氨酸的检测机制示意图

Figure 12. (a) Schematic diagram of the detection mechanism of probe 22 for glutamate and aspartic acid and its imaging of Asp/Glu in HeLa cells[113], Copyright 2012, American Chemical Society; (b) chemical structures of probe 23 and schematic diagram of the detection mechanism toward Glu and Asp

图13. (a~c)探针24~26的化学结构及其对谷氨酸、天冬氨酸及pH的检测机制示意图

Figure 13. (a~c) Chemical structures of probes 24~26 and schematic diagram of the detection mechanism toward Glu, Asp and pH

参考文献 122

[1]	Qi Y. L.; Jang D.; Ryu J.; Bai T. Y.; Shin Y.; Gu W.; Iyer A.; Li G.; Ma H. T.; Liou J.; Meer M.; Qiang Y.; Fang H. Nat. Commun. 2025, 16, 3300.
[2]	Pradhan T.; Jung H. S.; Jang J. H.; Kim T. W.; Kang C.; Kim J. S. Chem. Soc. Rev. 2014, 43, 4684. doi: 10.1039/c3cs60477b pmid: 24736802
[3]	Rizo J. Annu. Rev. Biophys. 2022, 51, 377.
[4]	Alves P. N.; Nozais V.; Hansen J. Y.; Corbetta M.; Nachev P.; Martins I. P.; Thiebaut de Schotten M. Nat. Commun. 2025, 16, 2555.
[5]	McCully B. H.; Hasan W.; Streiff C. T.; Houle J. C.; Woodward W. R.; Giraud G. D.; Brooks V. L.; Habecker B. A. Am. J. Physiol. Heart Circ. Physiol. 2013, 305, H1530.
[6]	Morales-Montor J.; Picazo O.; Besedovsky H.; Hernández-Bello R.; López-Griego L.; Becerril-Villanueva E.; Moreno J.; Pavón L.; Nava-Castro K.; Camacho-Arroyo I. Neuroimmunomodulation 2014, 21, 195. doi: 10.1159/000356521 pmid: 24504147
[7]	Matiushenko V.; Kutasevych Y.; Jafferany M. Dermatol. Ther. 2020, 33, e13337.
[8]	Naoi M.; Maruyama W.; Shamoto-Nagai M. J. Neural Transm. 2018, 125, 53.
[9]	Angelovski G.; Toth E. Chem. Soc. Rev. 2017, 46, 324. doi: 10.1039/c6cs00154h pmid: 28059423
[10]	Yang Y.; Sun L.; Liu X.; Liu W.; Zhang Z.; Zhou X.; Zhao X.; Zheng R.; Zhang Y.; Guo W.; Wang X.; Li X.; Pang J.; Li F.; Tao Y.; Shi D.; Shen W.; Wang L.; Zang J.; Li S. Biomed. Pharmacother. 2024, 176, 116844.
[11]	Lutz B.; Marsicano G.; Maldonado R.; Hillard C. J. Nat. Rev. Neurosci. 2015, 16, 705.
[12]	Akyuz E.; Polat A. K.; Eroglu E.; Kullu I.; Angelopoulou E.; Paudel Y. N. Life Sci. 2021, 265, 118826.
[13]	Lovinger D. M. Neuropharmacology 2010, 58, 951. doi: 10.1016/j.neuropharm.2010.01.008 pmid: 20096294
[14]	Pałasz A.; Menezes I. C.; Worthington J. J. Pharmacol. Rep. 2021, 73, 357. doi: 10.1007/s43440-021-00242-2 pmid: 33713315
[15]	Andersen J. V.; Markussen K. H.; Jakobsen E.; Schousboe A.; Waagepetersen H. S.; Rosenberg P. A.; Aldana B. I. Neuropharmacology 2021, 196, 108719.
[16]	Iovino L.; Tremblay M. E.; Civiero L. J. Pharmacol. Sci. 2020, 144, 151. doi: S1347-8613(20)30076-1 pmid: 32807662
[17]	Bukke V. N.; Archana M.; Villani R.; Romano A. D.; Wawrzyniak A.; Balawender K.; Orkisz S.; Beggiato S.; Serviddio G.; Cassano T. Int. J. Mol. Sci. 2020, 21, 7452.
[18]	Zhang K, Han Y, Zhang P, Zheng Y. Cheng A. Front. Cell. Neurosci. 2023, 17, 1166480.
[19]	Zhong X. Y.; Gu H. Y.; Lim J. Y.; Zhang P.; Wang G. F.; Zhang K.; Li X. W. IBRO Neurosci. Rep. 2025, 18, 476.
[20]	Wu Z.; Lin D.; Li Y. Nat. Rev. Neurosci. 2022, 23, 257.
[21]	Wang Y.; Qian Y.; Zhang L.; Zhang Z.; Chen S.; Liu J.; He X.; Tian Y. J. Am. Chem. Soc. 2023, 145, 2118. doi: 10.1021/jacs.2c07053 pmid: 36650713
[22]	Banerjee S.; McCracken S.; Hossain M. F.; Slaughter G. Biosensors 2020, 10, 101.
[23]	Park C.; Oh Y.; Shin H.; Kim J.; Kang Y.; Sim J.; Cho H. U.; Lee H. K.; Jung S. J.; Blaha C. D.; Bennet K. E.; Heien M. L.; Lee K. H.; Kim I. Y.; Jang D. P. Anal. Chem. 2018, 90, 13348.
[24]	Ma L.; Zhao T.; Zhang P.; Liu M.; Shi H.; Kang W. Anal. Biochem. 2020, 593, 113594.
[25]	Costa B. M. C.; Prado A. A.; Oliveira T. C.; Bressan L. P.; Munoz R. A. A.; Batista A. D.; da Silva J. A. F.; Richter E. M. Talanta 2019, 204, 353.
[26]	Zhang J.; Jaquins-Gerstl A.; Nesbitt K. M.; Rutan S. C.; Michael A. C.; Weber S. G. Anal. Chem. 2013, 85, 9889. doi: 10.1021/ac4023605 pmid: 24020786
[27]	Phan N. T. N.; Hanrieder J.; Berglund E. C.; Ewing A. G. Anal. Chem. 2013, 85, 8448. doi: 10.1021/ac401920v pmid: 23915325
[28]	Hammad L. A.; Neely M.; Bridge B.; Mechref Y. J. Sep. Sci. 2009, 32, 2369.
[29]	Wu D.; Sedgwick A. C.; Gunnlaugsson T.; Akkaya E. U.; Yoon J.; James T. D. Chem. Soc. Rev. 2017, 46, 7105.
[30]	Mei Y.; Liu Z.; Liu M.; Gong J.; He X.; Zhang Q.-W.; Tian Y. Chem. Commun. 2022, 58, 6657.
[31]	Han Y.; Li X.; Li D.; Chen C.; Zhang Q.-W.; Tian Y. ACS Sens. 2022, 7, 1036.
[32]	Zeng S.; Wang S.; Xie X.; Yang S.-H.; Fan J.-H.; Nie Z.; Huang Y.; Wang H.-H. Anal. Chem. 2020, 92, 15194. doi: 10.1021/acs.analchem.0c03764 pmid: 33136382
[33]	Liang R.; Broussard G. J.; Tian L. ACS Chem. Neurosci. 2015, 6, 84.
[34]	Zhou N.; Huo F.; Yin C. Coord. Chem. Rev. 2024, 518, 216062.
[35]	Wu Z. F.; Ke J. X.; Liu Y. S.; Sun P. M.; Hong M. C. Acta Chim. Sinica 2022, 80, 542 (in Chinese).
	(吴志芬, 柯建熙, 刘永升, 孙蓬明, 洪茂椿, 化学学报, 2022, 80, 542.) doi: 10.6023/A21120571
[36]	Wang X. W.; Song Y. F.; Wan J.; Shi Y.; Zhang Q.-W.; Tian Y. Anal. Chem. 2025, 97, 14594.
[37]	Liu X.; Yu B.; Shen Y.; Cong H. Coord. Chem. Rev. 2022, 468, 214609.
[38]	Chan J.; Dodani S. C.; Chang C. J. Nat. Chem. 2012, 4, 973.
[39]	Yan Y. H.; Liang P. Z.; Zou Y.; Yuan L.; Peng X. J.; Fan J. L.; Zhang X. B. Acta Chim. Sinica 2023, 81, 1642 (in Chinese).
	(闫英红, 梁平兆, 邹杨, 袁林, 彭孝军, 樊江莉, 张晓兵, 化学学报, 2023, 81, 1642.) doi: 10.6023/A23050243
[40]	Gu X.; Wang X.; Cai W.; Han Y.; Zhang Q.-W. ACS Sens. 2024, 9, 3387.
[41]	Chen C.; Pan Y.; Li D.; Han Y.; Zhang Q.-W.; Tian Y. Anal. Chem. 2022, 94, 6289. doi: 10.1021/acs.analchem.2c00415 pmid: 35412308
[42]	Meng Z. X.; Tian X. M.; Zhang T. F. Chin. J. Org. Chem. 2024, 44, 2441 (in Chinese).
	(孟子翔, 田秀梅, 张天富, 有机化学, 2024, 44, 2441.) doi: 10.6023/cjoc202403058
[43]	Li N.; Tan X. F.; Yang Q. L. Chin. J. Org. Chem. 2022, 42, 1375 (in Chinese).
	(李娜, 谭啸峰, 杨晴来, 有机化学, 2022, 42, 1375.) doi: 10.6023/cjoc202112022
[44]	Bade A.; Yadav P.; Zhang L.; Naidu Bypaneni R.; Xu M.; Glass T. E. Angew. Chem. Int. Ed. 2024, 63, e202406401.
[45]	Lelou E.; Corlu A.; Nesseler N.; Rauch C.; Mallédant Y.; Seguin P.; Aninat C. Cells 2022, 11, 1021.
[46]	Chang X.; Tse A. M.; Fayzullina M.; Albanese A.; Kim M.; Wang C. F.; Zheng Z.; Joshi R. V.; Williams C. K.; Magaki S. D.; Vinters H. V.; Jones J. O.; Haworth I. S.; Seidler P. M. Sci. Adv. 2025, 11, eadr8055.
[47]	Vallone D.; Picetti R.; Borrelli E. Neurosci. Biobehav. Rev. 2000, 24, 125. doi: 10.1016/s0149-7634(99)00063-9 pmid: 10654668
[48]	Suominen T.; Uutela P.; Ketola R. A.; Bergquist J.; Hillered L.; Finel M.; Zhang H.; Laakso A.; Kostiainen R. PLOS ONE 2013, 8, e68007.
[49]	Cai T.; Zhang W.; Charlton F.; Sun Y.; Chen Y.; Fu Y.; Zhang Q.; Liu S. Biosens. Bioelectron. 2025, 280, 117444.
[50]	Seto D.; Maki T.; Soh N.; Nakano K.; Ishimatsu R.; Imato T. Talanta 2012, 94, 36.
[51]	Suzuki Y. Sens. Actuators B Chem. 2017, 239, 383.
[52]	Suzuki Y. Sensors 2019, 19, 3928.
[53]	Zhang X.; Zhu Y.; Li X.; Guo X.; Zhang B.; Jia X.; Dai B. Anal. Chim. Acta 2016, 944, 51.
[54]	Zhao J.-H.; Liu G.-Y.; Wang S.; Lu S.-S.; Sun J.; Yang X.-R. Chin. J. Anal. Chem. 2020, 48, e20081.
[55]	Li Y.; Xie Y.; Qin Y. Sens. Actuators B Chem. 2014, 191, 227.
[56]	He M.; Zheng X. J. Mol. Liq. 2012, 173, 29.
[57]	Liu X.; Tian M.; Gao W.; Zhao J. J. Anal. Methods Chem. 2019, 2019, 6540397.
[58]	Chaicham A.; Sahasithiwat S.; Tuntulani T.; Tomapatanaget B. Chem. Commun. 2013, 49, 9287.
[59]	Ji W.; Miao A.; Liang K.; Liu J.; Qi Y.; Zhou Y.; Duan X.; Sun J.; Lai L.; Wu J.-X. Nature 2024, 633, 473.
[60]	Kagiampaki Z.; Rohner V.; Kiss C.; Curreli S.; Dieter A.; Wilhelm M.; Harada M.; Duss S. N.; Dernic J.; Bhat M. A.; Zhou X. H.; Ravotto L.; Ziebarth T.; Wasielewski L. M.; Sönmez L.; Benke D.; Weber B.; Bohacek J.; Reiner A.; Wiegert J. S.; Fellin T.; Patriarchi T. Nat. Methods 2023, 20, 1426.
[61]	Hettie K. S.; Liu X.; Gillis K. D.; Glass T. E. ACS Chem. Neurosci. 2013, 4, 918.
[62]	Hettie K. S.; Glass T. E. Chem.-Eur. J. 2014, 20, 17488.
[63]	Zhang L.; Liu X. A.; Gillis K. D.; Glass T. E. Angew. Chem. Int. Ed. 2019, 58, 7611. doi: 10.1002/anie.201810919 pmid: 30791180
[64]	Yue Y.; Huo F.; Yin C. Anal. Chem. 2019, 91, 2255.
[65]	Zhou N.; Huo F.; Yue Y.; Yin C. J. Am. Chem. Soc. 2020, 142, 17751.
[66]	Zhou N.; Yin C.; Yue Y.; Zhang Y.; Cheng F.; Huo F. Chem. Commun. 2022, 58, 2999.
[67]	Zhang Q.-W.; Tian Y. Sci. China Chem. 2023, 66, 923.
[68]	Yan H.; Wang Y.; Huo F.; Yin C. J. Am. Chem. Soc. 2023, 145, 3229.
[69]	Mao L.; Han Y.; Zhang Q.-W.; Tian Y. Nat. Commun. 2023, 14, 1419.
[70]	Han Y.; Mao L.; Zhang Q.-W.; Tian Y. J. Am. Chem. Soc. 2023, 145, 23832.
[71]	Zhang Q.-W.; Li D.; Li X.; White P. B.; Mecinović J.; Ma X.; Ågren H.; Nolte R. J. M.; Tian H. J. Am. Chem. Soc. 2016, 138, 13541.
[72]	Zhang Q.-W.;Elemans, H. J. A. A. W.; White, P.; Nolte, R. J. M. Chem. Commun. 2018, 54, 5586.
[73]	Li D.; Feng Z.; Han Y.; Chen C.; Zhang Q.-W.; Tian Y. Adv. Sci. 2022, 9, 2104790.
[74]	Li D.; Han Y.; Jiang Y.; Jiang G.; Sun H.; Sun Z.; Zhang Q.-W.; Tian Y. ACS Appl. Mater. Interfaces 2022, 14, 1807.
[75]	Qu D.-H.; Wang Q.-C.; Zhang Q.-W.; Ma X.; Tian H. Chem. Rev. 2015, 115, 7543.
[76]	Busschaert N.; Caltagirone C.; Van Rossom W.; Gale P. A. Chem. Rev. 2015, 115, 8038. doi: 10.1021/acs.chemrev.5b00099 pmid: 25996028
[77]	Li J.; Wang J.; Li H.; Song N.; Wang D.; Tang B. Z. Chem. Soc. Rev. 2020, 49, 1144.
[78]	Yin H.; Cheng Q.; Bardelang D.; Wang R. JACS Au 2023, 3, 2356.
[79]	Wu Y.; Tang M.; Wang Z.; Shi L.; Xiong Z.; Chen Z.; Sessler J. L.; Huang F. Nat. Commun. 2023, 14, 4927.
[80]	Huang F.; Liu J.; Li M.; Liu Y. J. Am. Chem. Soc. 2023, 145, 26983. doi: 10.1021/jacs.3c10295 pmid: 38032103
[81]	Assaf K. I.; Nau W. M. Acc. Chem. Res. 2023, 56, 3451.
[82]	He Y. Q.; Chen L.; He R. L.; Zhong K. L.; Tang L. J. Chin. J. Org. Chem. 2022, 42, 785 (in Chinese).
	(何雨晴, 陈琳, 贺瑞丽, 钟克利, 汤立军, 有机化学, 2022, 42, 785.) doi: 10.6023/cjoc202108024
[83]	Nilam M.; Karmacharya S.; Nau W. M.; Hennig A. Angew. Chem. Int. Ed. 2022, 61, e202207950.
[84]	Li M.-S.; Quan M.; Yang X.-R.; Jiang W. Sci. China Chem. 2022, 65, 1733.
[85]	Hu C.; Jochmann T.; Chakraborty P.; Neumaier M.; Levkin P. A.; Kappes M. M.; Biedermann F. J. Am. Chem. Soc. 2022, 144, 13084.
[86]	Dummert S. V.; Saini H.; Hussain M. Z.; Yadava K.; Jayaramulu K.; Casini A.; Fischer R. A. Chem. Soc. Rev. 2022, 51, 5175. doi: 10.1039/d1cs00550b pmid: 35670434
[87]	Guo Y. J.; Zhang M. P.; Gong Z. T.; Zhao Y. L.; Shi K. Q.; He L. L. Acta Chim. Sinica 2025, 83, 309 (in Chinese).
	(郭煜静, 张梦盼, 巩子彤, 赵云莉, 石康琦, 何磊良, 化学学报, 2025, 83, 309.) doi: 10.6023/A24110357
[88]	Zhao Y.; Mei Y.; Sun J.; Tian Y. J. Am. Chem. Soc. 2025, 147, 5025.
[89]	Zou L.; Liu Y.; Liu J. Biosens. Bioelectron. 2025, 279, 117392.
[90]	Luo C.; Chen Y.; Gu J.; Cai H.; Lin H.; Jin Z.; Huang C. Anal. Chem. 2024, 96, 9969. doi: 10.1021/acs.analchem.4c01309 pmid: 38847356
[91]	Zhao Y.; Mei Y.; Liu Z.; Sun J.; Tian Y. Nat. Commun. 2025, 16, 1848.
[92]	Ligneul R.; Mainen Z. F. Current Biology 2023, 33, R1216.
[93]	Che F.; Zhao X.; Zhang X.; Ding Q.; Wang X.; Li P.; Tang B. Acta Chim. Sinica 2023, 81, 1255 (in Chinese).
	(车飞达, 赵晓茗, 张馨, 丁琪, 王昕, 李平, 唐波, 化学学报, 2023, 81, 1255.) doi: 10.6023/A23040191
[94]	Hettie K. S.; Glass T. E. ACS Chem. Neurosci. 2016, 7, 21.
[95]	Yue L.; Huang H.; Lin W. Angew. Chem. Int. Ed. 2024, 63, e202407308.
[96]	Dong H.; Li M.; Yan Y.; Qian T.; Lin Y.; Ma X.; Vischer H. F.; Liu C.; Li G.; Wang H.; Leurs R.; Li Y. Neuron 2023, 111, 1564. doi: 10.1016/j.neuron.2023.02.024 pmid: 36924772
[97]	Zhang X.; Liu G.; Zhong Y.-N.; Zhang R.; Yang C.-C.; Niu C.; Pu X.; Sun J.; Zhang T.; Yang L.; Zhang C.; Li X.; Shen X.; Xiao P.; Sun J.-P.; Gong W. M. Nat. Commun. 2024, 15, 8296. doi: 10.1038/s41467-024-52585-y pmid: 39333117
[98]	Kielland N.; Vendrell M.; Lavilla R.; Chang Y.-T. Chem. Commun. 2012, 48, 7401.
[99]	Dey N.; Ali A.; Podder S.; Majumdar S.; Nandi D.; Bhattacharya S. Chem.-Eur. J. 2017, 23, 11891.
[100]	Becchetti A.; Grandi L. C.; Cerina M.; Amadeo A. Pharmacol. Res. 2023, 189, 106698.
[101]	Jing M.; Zhang P.; Wang G.; Feng J.; Mesik L.; Zeng J.; Jiang H.; Wang S.; Looby J. C.; Guagliardo N. A.; Langma L. W.; Lu J.; Zuo Y.; Talmage D. A.; Role L. W.; Barrett P. Q.; Zhang L. I.; Luo M.; Song Y.; Zhu J. J.; Li Y. Nat. Biotechnol. 2018, 36, 726. doi: 10.1038/nbt.4184
[102]	Rocha-Resende C.; da Silva A. M.; Prado M. A. M.; Guatimosim S. Am. J. Physiol. Cell Physiol. 2020, 320, C155.
[103]	Fantozzi N.; Pétuya R.; Insuasty A.; Long A.; Lefevre S.; Schmitt A.; Robert V.; Dutasta J.-P.; Baraille I.; Guy L.; Genin E.; Bégué D.; Martinez A.; Pinet S.; Gosse I. New J. Chem. 2020, 44, 11853. doi: 10.1039/d0nj02794d
[104]	Norouzy A.; Azizi Z.; Nau W. M. Angew. Chem. Int. Ed. 2015, 54, 792. doi: 10.1002/anie.201407808 pmid: 25430503
[105]	Mei Y.; Zhang Q.-W.; Gu Q.; Liu Z.; He X.; Tian Y. J. Am. Chem. Soc. 2022, 144, 2351.
[106]	Zhou Y.; Yoon J. Chem. Soc. Rev. 2012, 41, 52.
[107]	Xu M.; Yadav P.; Liu X.; Gillis K. D.; Glass T. E. ACS Chem. Neurosci. 2025, 16, 1238.
[108]	He L.; So V. L. L.; Xin J. H. Sens. Actuators B Chem. 2014, 192, 496.
[109]	Dávalos A.; Castillo J.; Serena J.; Noya M. Stroke 1997, 28, 708. doi: 10.1161/01.str.28.4.708 pmid: 9099183
[110]	Skvortsova V. I.; Raevskii K. S.; Kovalenko A. V.; Kudrin V. S.; Malikova L. A.; Sokolov M. A.; Alekseev A. A.; Gusev E. I. Neurosci. Behav. Physiol. 2000, 30, 491.
[111]	Niebroj-Dobosz I.; Janik P. Acta Neurol. Scand. 1999, 100, 6. pmid: 10416506
[112]	D'Aniello, A.; Lee, J. M.; Petrucelli, L.; Di Fiore, M. M. Neurosci. Lett. 1998, 250, 131. pmid: 9697936
[113]	Guria S.; Ghosh A.; Manna K.; Pal A.; Adhikary A.; Adhikari S. Dyes Pigments 2019, 168, 111.
[114]	Dessingou J.; Mitra A.; Tabbasum K.; Baghel G. S.; Rao C. P. J. Org. Chem. 2012, 77, 371.
[115]	Ranjani M.; Kalaivani P.; Dallemer F.; Selvakumar S.; Kalpana T.; Prabhakaran R. Inorg. Chim. Acta 2022, 530, 120683.
[116]	Klockow J. L.; Hettie K. S.; Glass T. E. ACS Chem. Neurosci. 2013, 4, 1334.
[117]	Hettie K. S.; Klockow J. L.; Glass T. E. J. Am. Chem. Soc. 2014, 136, 4877.
[118]	Yin C.; Huo F.; Cooley N. P.; Spencer D.; Bartholomew K.; Barnes C. L.; Glass T. E. ACS Chem. Neurosci. 2017, 8, 1159.
[119]	Wang X.; Chen C.; Tian Y.; Zhang Q.-W. J. Am. Chem. Soc. 2025, 147, 17994.
[120]	Song Y.; Tong X.; Han Y.; Zhang Q.-W. Aggregate 2025, 6, e680.
[121]	Wen B.; Zhang W.; Zhang Q.-W.; Tian Y. Chin. Chem. Lett. 2025, 111459.
[122]	Jiang L.; Chen Z. H.; Fan Y. Acta Chim. Sinica 2024, 82, 1069 (in Chinese). doi: 10.6023/A24070218
	(蒋励, 陈子晗, 凡勇, 化学学报, 2024, 82, 1069.) doi: 10.6023/A24070218

用于神经递质检测与生物成像的有机小分子荧光探针^★

Organic Small-Molecule Fluorescent Probes for Neurotransmitter Sensing and Bioimaging^★

RichHTML

PDF

可视化

摘要/Abstract

引用此文

分享此文

图/表 13

参考文献 122

相关文章 15

编辑推荐

相关统计

本文评价

[1]	赵玉强, 那迪, 和晓波, 朱立平, 周莹, 曾广智, 樊保敏. 无需催化剂的光介导生物正交荧光探针实例[J]. 化学学报, 2025, 83(8): 816-826.
[2]	潘禹州, 殷炜杰, 张雨霞. 手性超分子组装圆偏振室温磷光材料的研究进展[J]. 化学学报, 2025, 83(6): 639-654.
[3]	李菲, 伊思静, 王姣, 刘晓霞, 高文梅, 李婧婧, 张海峰. 稀土超分子荧光有序聚集体在离子液体中聚集及荧光性能的研究[J]. 化学学报, 2025, 83(5): 453-462.
[4]	郭煜静, 张梦盼, 巩子彤, 赵云莉, 石康琦, 何磊良. 基于大环主体分子的核酸探针设计策略及生物应用[J]. 化学学报, 2025, 83(3): 309-318.
[5]	刘童, 皮慧慧, 陈冰昆, 张小玲. 近红外二区硫系银量子点制备方法及癌症诊疗应用研究进展[J]. 化学学报, 2024, 82(9): 1001-1012.
[6]	王丹钰, 郭子涵, 郭梦珂, 易桦, 黄梦雨, 段捷, 张开翔. DNA纳米花生物医学研究进展概述[J]. 化学学报, 2024, 82(6): 677-689.
[7]	王博, 蔡向东, 肖建喜. 肿瘤特异性多肽探针及其在生物成像中的应用[J]. 化学学报, 2024, 82(3): 367-376.
[8]	王敏, 陈帮塘, 陈桥林, 王俊, 陈名钊, 蒋志龙, 王平山. 6,6″-二(2,6-二甲氧基苯)-三联吡啶及其衍生物金属配位自组装的研究进展[J]. 化学学报, 2024, 82(3): 336-347.
[9]	蒋励, 陈子晗, 凡勇. 近红外二区荧光探针在活体多重成像中的研究进展[J]. 化学学报, 2024, 82(10): 1069-1085.
[10]	车飞达, 赵晓茗, 张馨, 丁琪, 王昕, 李平, 唐波. 抑郁症相关活性分子的荧光成像^★[J]. 化学学报, 2023, 81(9): 1255-1264.
[11]	洪梅, 高金强, 李彤, 杨世和. 原位刻蚀调控多级孔分子筛策略及其应用进展^★[J]. 化学学报, 2023, 81(8): 937-948.
[12]	李永雪, 刘育. 基于两亲性杯[4]芳烃的超分子二级组装及其生物应用^★[J]. 化学学报, 2023, 81(8): 928-936.
[13]	武虹乐, 郭锐, 迟涵文, 唐永和, 宋思睿, 葛恩香, 林伟英. 喹啉基粘度荧光探针的合成及其检测应用[J]. 化学学报, 2023, 81(8): 905-911.
[14]	谌业勤, 陈金平, 于天君, 曾毅, 李嫕. 多糖基质诱导有机小分子室温磷光研究[J]. 化学学报, 2023, 81(5): 450-455.
[15]	贺晓梦, 袁方, 张素雅, 张健健. 基于尼罗红类ONOO^–近红外荧光探针的开发及其成像应用[J]. 化学学报, 2023, 81(11): 1515-1521.

用于神经递质检测与生物成像的有机小分子荧光探针★

Organic Small-Molecule Fluorescent Probes for Neurotransmitter Sensing and Bioimaging★

RichHTML

PDF

可视化

摘要/Abstract

引用此文

分享此文

图/表 13

参考文献 122

相关文章 15

编辑推荐

相关统计

本文评价

用于神经递质检测与生物成像的有机小分子荧光探针^★

Organic Small-Molecule Fluorescent Probes for Neurotransmitter Sensing and Bioimaging^★