有机化学 ›› 2001, Vol. 21 ›› Issue (4): 273-277. 上一篇    下一篇

研究论文

酶催化脱保护方法及在生物有机化学中的应用

陈忠周;李艳梅;赵刚;赵玉芬   

  1. 清华大学生命科学院;生命有机磷教育部重点实验室
  • 发布日期:2001-04-25

Application of enzymatic deprotection reactions to bioorganic chemistry

Chen Zhongzhou;Li Yanmei;Zhao Gang;Zhao Yufen   

  • Published:2001-04-25

系统地介绍和评述了近年来氨基、羧基、巯基和羟基等到基团的酶催化脱保护方法的进展。由于酶催化脱保护方法具有反应条件温和(中性,水溶液)、选择性和专一性高、副反应少等到优点,故在多肽缀合物、糖等敏感多官能团化合物的研究中有很广阔的应用前景。

关键词: 酶催化, 脱保护, 生物有机化学, 氨基, 羧基, 巯基, 羟基, 生物催化, 多肽缀合物, 保护基

Recent advances is enzymatic deprotection reactions of amino groups, carboxyl groups, thiol groups and hydroxyl groups are reviewed. Enzymatic deprotection techniques, which have the advantages of high selectivity, high specificity under mild conditions and in aqueous solutions, are being evaluated as potential methods for synthesizing the sensitive compounds such as peptide conjugates and carbohydrates.

Key words: ENZYME CATALYSIS, AMINO GROUP, CARBOXY GROUP, MERCAPTO GROUP, HYDROXY GROUP, BIOCATALYSIS, PROTECTIVE GROUP

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