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有机化学
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有机化学 ›› 2010, Vol. 30 ›› Issue (04): 551-557. 上一篇    下一篇

研究论文

非水介质中曲克芦丁乙烯酯的酶促合成

肖咏梅*,1，栗俊田1，毛璞1，赵贞1，林贤福2   

  1. (1河南工业大学化学化工学院 郑州 450001)
    (2浙江大学化学系 杭州 310027)
  • 收稿日期:2009-08-06 修回日期:2009-10-29 发布日期:2010-04-28
  • 通讯作者: 肖咏梅 E-mail:henangongda@yahoo.com
  • 基金资助:

    河南省基础与前沿技术研究课题[072300420070]；河南省科技攻关[82102330014]

Enzymatic Synthesis of Vinyl Troxerutin Esters in Nonaqueous Medium

Xiao Yongmei*,1 Li Juntian1 Mao Pu1 Zhao Zhen1 Lin Xianfu2   

  1. (1 College of Chemistry & Chemical Engineering, Henan University of Technology, Zhengzhou 450001)
    (2 Department of Chemistry, Zhejiang University, Hangzhou 310027)
  • Received:2009-08-06 Revised:2009-10-29 Published:2010-04-28
  • Contact: XIAO Yong-Mei E-mail:henangongda@yahoo.com

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1279

被引次数

4 | 1

在非水介质中, 选择性催化曲克芦丁羟乙基上的伯羟基发生酯交换反应, 合成一系列曲克芦丁乙烯酯. 同时考察了酶源、酶量、反应介质、酰化试剂链长、溶剂含水量、反应时间等因素对曲克芦丁酶促酯交换反应区域选择性的影响. 结果表明: 以吡啶为溶剂, 其含水量＜1%时, 枯草杆菌碱性蛋白酶催化活性最高, 随着酰基供体碳链的减少, 产率增大, 且反应达平衡的时间缩短.

关键词: 曲克芦丁, 酶促合成, 区域选择性, 酯交换反应

Through regioselective acylation of the primary hydroxyl groups in troxerutin, a series of vinyl troxerutin esters were synthesized in nonaqueous medium. The influence parameters such as enzyme source, the amount of the enzyme, solvents, chain length of acyl donors, water content of solvents and reaction time were investigated. The results indicated that alkaline protease from Bacillus subtilis performed highest activity in pyridine with water content lower than 1%. As decreasing of the chain length of the acyl donor, the transesterification reached equilibrium more rapidly and yields were improved.

Key words: troxerutin, enzymatic synthesis, regioselective, transesterification