有机化学 ›› 2021, Vol. 41 ›› Issue (3): 1200-1206.DOI: 10.6023/cjoc202009016 上一篇    下一篇

研究论文

基于硝基还原机理的一氧化碳荧光探针的开发及细胞成像研究

陈恩庆a, 唐永和a, 王蕾b, 任江波a, 林伟英a,*()   

  1. a 广西电化学能源材料重点实验室 广西大学光功能材料与化学生物学研究院 广西大学化学化工学院 南宁 530004
    b 南宁市食品药品检验所 南宁 530007
  • 收稿日期:2020-09-07 修回日期:2020-10-06 发布日期:2020-10-28
  • 通讯作者: 林伟英
  • 基金资助:
    国家自然科学基金(21672083); 国家自然科学基金(21877048); 国家自然科学基金(22077048); 广西大学启动基金(A3040051003); 广西省自然科学基金(2019GXNSFBA245068)

Development of a New Carbon Monoxide Fluorescent Probe Based on Nitro Reduction and Its Bioimaging Research in Living Cells

Enqing Chena, Yonghe Tanga, Lei Wangb, Jiangbo Rena, Weiying Lina,*()   

  1. a Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004
    b Nanning Institute for Food and Drug Control, Nanning 530007
  • Received:2020-09-07 Revised:2020-10-06 Published:2020-10-28
  • Contact: Weiying Lin
  • About author:
    * Corresponding author. E-mail:
  • Supported by:
    National Natural Science Foundation of China(21672083); National Natural Science Foundation of China(21877048); National Natural Science Foundation of China(22077048); Startup Fund of Guangxi University(A3040051003); Natural Science Foundation of Guangxi Province(2019GXNSFBA245068)

一氧化碳(CO)作为一种重要的细胞气体信号传递分子, 参与生物体内重要的生理和病理功能. 选用经典的荧光平台——萘酰亚胺荧光平台, 以硝基基团作为识别位点, 通过严谨的设计和两步合成的方法, 开发了一种新型用来检测一氧化碳的荧光增强型荧光探针NaLy-CO. 光谱测试数据表明, 该荧光探针对一氧化碳具有较好的选择性和灵敏度. 利用荧光共聚焦成像技术, 成功地对细胞外源性和内源性一氧化碳进行了检测. 此外, 共定位成像实验表明, 该荧光探针可以用于检测细胞溶酶体中的一氧化碳.

关键词: 一氧化碳, 荧光探针, 生物成像, 硝基, 萘酰亚胺

Carbon monoxide (CO), as an important cell signaling molecule, is involved in important physiological and pathological functions in organism. A new fluorescence enhanced CO fluorescent probe NaLy-CO was developed by using naphthalimide dye as fluorescence platform and nitro group as the recognition site. The results of spectrum test in solution showed that the fluorescent probe NaLy-CO had good selectivity and sensitivity to CO. The fluorescent probe NaLy-CO was successfully used to detect exogenous and endogenous CO in living HeLa cells by using confocal fluorescence imaging technology. In addition, the colocation imaging experiments showed that the fluorescent probe NaLy-CO could detect CO in the lysosome of living cells.

Key words: carbon monoxide, fluorescent probe, bioimaging, nitro, naphthalimide